LOCUS       sequence-43469-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    436..469
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aacgaattcg cccttatata tttaattaaa gatcaaccgg tctcaacaaa cttcccatac
       61 taaagctttc tcttgatata tagtcaaact gaatgtatac tgaaacacat attcaaggca
      121 ttgattttaa atgcacttga accgttattc catttctttt tctactcatc atttattgtc
      181 gccctttacg aggtattttt tctttttcga gtgaaaaatt ttaaagaagc gatgagctaa
      241 ggtctattta gctttatgaa aagaaaacat tatatcactt actgctttac tttagacatt
      301 cctctccaaa gtgattattt taaagattca aagtacctct gagttaattt gacatcgaat
      361 tctactctta ttattttaag gtacctctac tttacgcggt cacatctcga gggctgcagg
      421 tcgacaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctagag
      481 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
      541 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
      601 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
      661 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
      721 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aagg
//
