LOCUS       sequence-43470-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ251 (pKAN 24xPP7SL cyc)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..381)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(436..469)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(520..767)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     733..751
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     769..785
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     770..786
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
       61 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
      121 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
      181 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
      241 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
      301 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
      361 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg caagctaaac
      421 agatctctag acctaataac ttcgtatagc atacattata cgaagttata ttaagggttg
      481 tcgacctgca gcgtacgaag cttcagctgg gtaccggccg caaattaaag ccttcgagcg
      541 tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta
      601 cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa
      661 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
      721 tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc gaggtcgacg
      781 gtatcgataa gcttgatatc gaattcctgc agcccggggg atctaatgaa cccgggaata
      841 ctgcagccag cgagcccata tgctctgctg gtttctagag tcgacctgca gggagcgacg
      901 ccatatcgtc tgctcctt
//
