LOCUS       sequence-434709-        2695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondrial cytosine base editor arm in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: TL1 alphaDdCBE 2 (left, DddA11-C)
FEATURES             Location/Qualifiers
     source          1..2695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2293..2544
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg acatcgcgga tctgcgtacc
      181 ctgggttaca gccagcagca gcaggagaag atcaagccaa aggtgcgcag caccgtggcc
      241 cagcaccatg aagctctggt gggtcacggc ttcacccacg cgcacatcgt ggctctgagc
      301 cagcacccag ccgcgctggg taccgtggcc gtgaaatatc aggacatgat tgctgccctg
      361 ccagaggcta cccatgaagc gattgtgggt gtgggcaaga gaggagccgg tgctcgtgcg
      421 ctggaggccc tgctgaccgt ggccggtgaa ctgcgtggcc cgccgctgca gctggatacc
      481 ggccagctgc tgaaaatcgc gaaacgtggc ggtgtgaccg ctgtggaagc tgtgcatgcc
      541 tggcgtaatg ctctgaccgg tgcccccctg aacctgaccc cggaccaagt ggtggctatc
      601 gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      661 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa caatggcggc
      721 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
      781 accccggacc aagtggtggc tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg
      841 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      901 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
      961 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt
     1021 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
     1081 ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc
     1141 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
     1201 gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg
     1261 ttgccggtgc tgtgccagga ccatggcctc acacccgatc aggtcgtggc tatcgccagc
     1321 aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag
     1381 gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa
     1441 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg
     1501 gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag
     1561 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
     1621 gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
     1681 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa caatggcggc
     1741 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
     1801 accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg
     1861 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
     1921 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
     1981 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat
     2041 ggcggcaagc aagcgctcga aagcattgtg gcccagctga gccggcctga tccggcgttg
     2101 gccgcgttga ccaacgatca cctggtggcc ctggcctgcc tgggcggtcg tccggccctg
     2161 gatgcggtga agaaaggcct gggtggatcc gccattccag tgaagcgcgg cgctaccggt
     2221 gaaaccaaag tgtttatcgg taacagcaac agcccgaaga gcccgaccaa aggcggttgc
     2281 tctggcggct caactaatct gagcgacatc attgagaagg agactgggaa acagctggtc
     2341 attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca
     2401 gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg
     2461 ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc
     2521 gagaataaga tcaagatgct gtgataaggg gtctttgtcc tctgtactgt ctctctcctt
     2581 gcccctaacc caaaaagctt catttttctg tgtaggctgc acaagagcct tgattgaaga
     2641 tatattcttt ctgaacagta tttaaggttt ccaataaaat gtacacccct cagaa
//
