LOCUS       sequence-43471-e         308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
FEATURES             Location/Qualifiers
     source          1..308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     155..173
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
       61 atcggggatg tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga
      121 caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg
      181 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat
      241 ggagccctcc atgcgcacct tgaagcgcat gaactctttg atgacggcca tgttgttgtc
      301 ctcggaag
//
