LOCUS       sequence-43472-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ276 (pURA MET28 PCP-2x-yeGFP)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(31..747)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(34..747)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aactcaatgg tgatggtgat gatgaccggt ttatttgtac aattcatcca taccatgggt
       61 aataccagca gcagtaacaa attctaacaa gaccatgtgg tctctctttt cgtttggatc
      121 tttggataag gcagattgag tggataagta atggttgtct ggtaacaaga ctggaccatc
      181 accaattgga gtattttgtt gataatggtc agctaattga acagaaccat cttcaatgtt
      241 gtgtctaatt ttgaagttaa ctttgatacc attcttttgt ttgtcagcca tgatgtaaac
      301 attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccat cttctttaaa
      361 atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact tgacttcagc
      421 tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa cataaccttc
      481 tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc tagaaaaaca
      541 ttgaacacca taagttaaag tagtgactaa ggttggccat ggaactggca atttaccagt
      601 agtacaaata aattttaagg tcaatttacc gtaagtagca tcaccttcac cttcaccgga
      661 gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc
      721 agtgaataat tcttcacctt tagacatgtc gacggtatcg ataagcttga tatcgaattc
      781 ctgcagcccg ggggatcttt tgtacaattc atccatacca tgggtaatac cagcagcagt
      841 aacaaattct aacaagacca tgtgg
//
