LOCUS       sequence-43477-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases.
  JOURNAL   Mol Cell Biol. 1999 Feb . 19(2):1569-81.
  PUBMED    9891090
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Feb . 19(2):1569-81."
COMMENT     Sequence Label: pCDNA3 MKK7b1(K149A)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(222..233)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tactagagac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttcgg cagccaacat ggactagatg acaaaatggc ggcgtcctcc ctggagcaga
      121 agctgtcccg cctggaagcc aagctgaagc aggagaaccg tgaggcccgc aggaggatcg
      181 acctcaactt ggatatcagc ccacagcggc ccaggcccac cctgcaactc ccactggcca
      241 acgatggggg cagccgctca ccatcctcag agagctcccc acagcaccct acacccccca
      301 cccggccccg ccacatgctg gggctcccat caaccttgtt cacaccgcgc agtatggaga
      361 gcatcgagat tgaccagaag ctgcaggaga tcatgaagca gacagggtac ctgactatcg
      421 ggggccagcg ttatcaggca gaaatcaatg acttggagaa cttgggtgag atgggcagtg
      481 gtacctgtgg tcaggtgtgg aagatgcggt tccggaagac aggccacatc attgctgttg
      541 cgcaaatgcg gcgctctggg aacaaggaag agaataagcg cattttgatg gacctggatg
      601 tagtactcaa gagccatgac tgcccttaca tcgttcagtg ctttggcacc ttcatcacca
      661 acacagacgt ctttattgcc atggagctca tgggcacatg tgcagagaag ctgaagaaac
      721 gaatgcaggg ccccattcca gagcgaatcc tgggcaagat gactgtggcg attgtgaaag
      781 cactgtacta tctgaaggag aagcatggcg tcatccatcg cgatgtcaaa ccctccaaca
      841 tcctgctaga tgagcggggc cagatcaagc
//
