LOCUS       sequence-43481-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Scheich C, Niesen FH, Seckler R, Bussow K
  TITLE     An automated in vitro protein folding screen applied to a human 
            dynactin subunit.
  JOURNAL   Protein Sci. 2004 Feb . 13(2):370-80.
  PUBMED    14739323
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2004 Feb . 13(2):370-80."
COMMENT     Sequence Label: pQTEV
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(61..155)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(229..249)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(274..294)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             307..318
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(361..367,368..384,385..390,391..405))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    369..385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(429..447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        515..619
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgccaa gctagcttgg
       61 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      121 ttctgaggtc attactggat ctatcaacag gagtccaagc tcagctaatt aagcttggct
      181 gcaggtcgag cggccgcggt accgagatct atcaacagtc gacccggatc cctgaaaata
      241 aagattctca gtagtgggga tgtcgtaatc gctatggtga tggtgatggt gatgtttcat
      301 agttaatttc tcctctttaa tgaattctgt gtgaaattgt tatccgctca caattgaatc
      361 tattataatt gttatccgct cacaaagcaa ataaattttt tatgatttct cgaggtgaag
      421 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      481 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      661 tgcggcattt tgcccttcct gtttt
//
