LOCUS       sequence-434844-        2768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc luminescence reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-872
FEATURES             Location/Qualifiers
     source          1..2768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..56
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(691..709)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        777..881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1103..1894
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2065..2653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2554..2573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
       61 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac
      121 tctgtggtct caaggtatgg tgttcacgct ggaggacttc gtgggcgact ggcgccagac
      181 ggcgggctac aacctggacc aggtgctgga gcagggcggc gtgagcagcc tgttccagaa
      241 cctgggcgtg agcgtgacgc cgatccagcg catcgtgctg agcggcgaga acggcctgaa
      301 gatcgacatc cacgtgatca tcccgtacga gggcctgagc ggcgaccaga tgggccagat
      361 cgagaagatc ttcaaggtgg tgtacccggt ggacgaccac cacttcaagg tgatcctgca
      421 ctacggcacg ctggtgatcg acggcgtgac gccgaacatg atcgactact tcggccgccc
      481 gtacgagggc atcgcggtgt ttgacggcaa gaagatcacg gtgacgggca cgctgtggaa
      541 cggcaacaag atcatcgacg agcgcctgat caacccggac ggcagcctgc tgttccgcgt
      601 gacgatcaac ggcgtgacgg gctggcgcct gtgcgagcgc atcctggcgg gttcgtgaga
      661 ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta
      721 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      781 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      841 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt
      901 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
      961 atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1021 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1081 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1141 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1201 gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac
     1261 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1321 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1381 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1441 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1501 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1561 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     1621 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     1681 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     1741 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     1801 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt
     1861 aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat
     1921 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2761 ctttgagt
//
