LOCUS       sequence-434846-        2970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mVenus fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-860
FEATURES             Location/Qualifiers
     source          1..2970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(237..239,240..953)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(993..1011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1079..1183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1405..2196
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2367..2955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2856..2875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
       61 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      181 aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct cattcggtga
      241 gcaagggcga ggagctgttc accggcgtgg tgcccatcct ggtggagctg gacggcgacg
      301 tgaacggcca caagttcagc gtgagcggcg agggcgaggg cgacgccacc tacggcaagc
      361 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctggtga
      421 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      481 acttcttcaa gagcgccatg cccgagggct acgtgcagga gcgcaccatc ttcttcaagg
      541 acgacggtaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      601 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg
      661 agtacaacta caacagccac aacgtgtaca tcaccgccga caagcagaag aacggcatca
      721 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctg gccgaccact
      781 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      841 gctaccagag caagctgagc aaggacccca acgagaagcg cgaccacatg gtgctgctgg
      901 agttcgtgac cgccgccggc atcaccctgg gcatggacga gctgtacaag taagctttga
      961 gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat ttttataggt
     1021 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     1081 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1141 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct cacgcaactg
     1201 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1261 ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     1321 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     1381 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     1441 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1501 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt
     1561 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1621 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1681 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1741 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1801 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1861 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1921 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1981 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     2041 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     2101 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac
     2161 gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat
     2221 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2281 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2341 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2401 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2461 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2521 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2581 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2641 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2701 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2761 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2821 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2881 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2941 cggagcctat ggaaaaacgc cagcaacgcg
//
