LOCUS       sequence-434854-       12505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted removal of m6A on Malat1 at 
            the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under 
            human Syn1 and U6 promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Madugalle SU, Liau WS, Zhao Q, Li X, Gong H, Marshall PR, 
            Periyakaruppiah A, Zajaczkowski EL, Leighton LJ, Ren H, Musgrove 
            MRB, Davies JWA, Kim G, Rauch S, He C, Dickinson BC, Fulopova B, 
            Fletcher LN, Williams SR, Spitale RC, Bredy TW
  TITLE     Synapse-Enriched m(6)A-Modified Malat1 Interacts with the Novel 
            m(6)A Reader, DPYSL2, and Is Required for Fear-Extinction Memory.
  JOURNAL   J Neurosci. 2023 Oct 25;43(43):7084-7100. doi: 
            10.1523/JNEUROSCI.0943-23.2023. Epub 2023 Sep 5.
  PUBMED    37669863
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0943-23.2023"; journalName: "J Neurosci"; date: 
            "2023-10-25- 25"; volume: "43"; issue: "43"; pages: "7084-7100"
COMMENT     Sequence Label: CIRTS-FTO-Malat1
FEATURES             Location/Qualifiers
     source          1..12505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4236..4262
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     RBS             4499..4507
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        6853..7101
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6853..6873
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7024..7043
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7197..7785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7250..7270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7668..7679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7788..7804)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7789..7805)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8310..8490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8516..8533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8522..8746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8792..9220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8879..8898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9089..9110
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9229..9249)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9234..9563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9414..9549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9476..9495
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9611..9658
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9677..10051
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10181..10302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10218..10237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10272..10291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10351..10367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10351..10367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10364..10386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10375..10391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10399..10405,10406..10423,10424..10429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10444..10465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10582..10599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10753..11341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10833..10852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11512..12303,12304..12372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12135..12154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12373..12477)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtatggg aattatcaac acccttcaaa aatactactg tcgcgtccgt gggggtcgtt
     3781 gcgcagtcct gtcgtgcctg cctaaagagg agcagatcgg taaatgctca acccgtggac
     3841 gtaagtgctg tcgtcgcaag aaaggcggtt ctggaggctc aggggggagc ggagggtccg
     3901 gtggatcagg aggctccatg gcagttcccg agacccgccc taaccacact atttatatca
     3961 acaacctcaa tagcaagatc aagaaggatg agctaaaaaa gtccctgtac gccatcttct
     4021 cccagtttgg ccagatcctg gatatcctgg taccgcggca gcgtaccccg aggggccagg
     4081 cctttgtcat cttcaaggag gtcagcagcg ccaccaacgc cctgcgctcc atgcagggtt
     4141 tccctttcta tgacaaacct atgcgtatcc agtatgccaa gaccgacaaa cgtatcccgg
     4201 ccaagatgaa aggcaccttc gtgggatccc ttcaactgcc tccacttgaa agactgacac
     4261 tgggcggtag tggaggaagc ggggggagcg gagggagtgg tggatccggc ggctccaagc
     4321 gcgtccagac cgcggaggaa cgagagcggg aagctaagaa actgaggctc cttgaggagc
     4381 ttgaagacac ttggcttcct tacctgaccc ccaaagatga tgagttctat cagcagtggc
     4441 agctgaaata ccctaaactg gttttccgag aggccggcag cataccagag gagctgcata
     4501 aggaggtccc cgaggccttt ctcacactgc ataagcatgg ctgcttgttt cgggacgtgg
     4561 tgaggatcca aggcaaagat gtgctcaccc cagtgtctcg catcctcatc ggggacccag
     4621 gctgcaccta caagtacttg aacaccagac tcttcacggt gccctggccc gtgaagggct
     4681 gcacggtcaa gtacacagag gctgagatcg ccgctgcatg tcagaccttc ctaaagctca
     4741 atgactacct ccaggtggag accatccagg ccttggaaga actggctgtc agagagaagg
     4801 ccaatgaaga cgctgtgcca ctgtgcatgg cagagttccc cagggccggc gtggggccgt
     4861 cctgcgatga tgaagtggac cttaagagca gagcagccta caacgtgact ttgctaaact
     4921 tcatggatcc tcagaagatg ccctacttga aagaggagcc ctatttcggc atggggaaga
     4981 tggcggtgag ctggcatcac gatgagaacc tggtggacag gtcagccgtg gcagtgtaca
     5041 gctatagctg cgaaggctct gaggatgaaa gtgaggacga gtccagcttc gaaggcagag
     5101 atcctgatac ttggcatgtt ggttttaaga tctcttggga catcgagaca ccaggattaa
     5161 caatccctct tcaccaggga gactgctatt tcatgctgga tgacctcaat gccacccacc
     5221 agcactgtgt tttggctggc tcacagcctc ggtttagttc cactcaccgt gtggcagagt
     5281 gctcaacagg caccttggat tatatcttag aacgctgtca gttggcgctg cagaatgtcc
     5341 tcaatgactc agacgatggc gacgtctcgt tgaaatcctt tgatcctgca gttttgaaac
     5401 aaggagagga aatccataat gaggtggagt ttgagtggct gaggcagttc tggtttcaag
     5461 gcaatcgata caaactttgc accgattggt ggtgtgagcc catgactcac ctggaggggc
     5521 tgtggaagaa gatggagagc atgacaaatg cggtgctccg tgaagttaaa agagaggggc
     5581 tcccggtgga acaaaggagt gagattctgt ctgccatcct ggtcccgctc accgtgcgcc
     5641 agaacctgag gaaggagtgg catgccaggt gccagtcccg agtcgtccgg actttaccag
     5701 tacagcagaa accagactgc cggccatatt gggagaagga tgacccttcc atgcctctgc
     5761 cctttgacct cacagacgtg gtttccgagc tcagaggcca gctgctggaa gcaagatcct
     5821 aggctagcgg gagcctctgc cctcctcctg cagcccggat ggctctcctc tgtttttggt
     5881 ttgcttcctt ttgtttgccc ttcttttggg tgaccggggt ggccaccatt cctgtccctg
     5941 cccagtgggg aggggagcaa ggccttctgc caagaggagg agcatgtccc ctgccactgt
     6001 tgcatgcacc ccggccctgt gactctgtgt gcaagcccag cagcctgtgg ggcagggtgc
     6061 caagagggga cgggacggac gttccaggac agagggagtc gaggatgtga tccaggacgg
     6121 gtggggggtg gaagggtggg aaagggcagg aaggccttgg aagctaacat gctttcacta
     6181 ctggaggggg acggatagag cggatggcac tcgggtctca ctgacgctgt tctgaaacca
     6241 tctggctggc tttctgaggt caggctgggt agggggctgt catcggccat actgctgatc
     6301 acacttgccc tcatccgtcc agcgatctca cgctgttctg taaatacctg gtgctaacat
     6361 cccatgccgc tcccccatgg cacccccagc ctcctgccca gttcaggctg gtccaggaat
     6421 ggatgtgggg gatggtcgct gtgtaacgtg ctggtgatct ttttccccta ccccttttat
     6481 gacccttaaa actttgattt tgtcctaaac atgccgggcc aactaaaggg tggggagagg
     6541 agagacgagc cccaccatgc tcacaagaac caacctgcaa taaacacttc tgtgggccgc
     6601 cgcgcagagc acgcagcggg cacctcctgc ccctccatgg cgacttggca tcgctttctg
     6661 cccgcctctg ccctcgccct gcctgcccca ccccccagcg gagagcatga tccgcgccct
     6721 tgcttctgac tctagcctct gggacacatc agtcaacgtg ggcagttcag tctgggttcg
     6781 ttcctttctc tgttctcatc tggcccccca gggcgatctt gttgcttttt gctgggacct
     6841 gcccagtcta gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     6901 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     6961 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     7021 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     7081 ttgtggaaag gacgaaacac cggccagatc tgagcctggg agctctctgg ccttattcca
     7141 aatactagcc taaccttttt gttttttttt ttgaattcga tatcaagctt atcgataatc
     7201 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7261 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7321 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7381 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7441 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7501 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7561 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7621 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7681 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7741 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7801 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7861 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     7921 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7981 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     8041 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     8101 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     8161 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     8221 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     8281 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     8341 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8401 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8461 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     8521 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8581 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     8641 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     8701 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8761 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     8821 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8881 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8941 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9001 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9061 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9121 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9181 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     9241 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9301 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9361 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9481 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     9541 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     9601 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     9661 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     9721 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     9781 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     9841 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     9901 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     9961 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
    10021 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
    10081 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    10141 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
    10201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    10321 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10381 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10441 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    11161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    11221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    11281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    11341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    11401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    12001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    12121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    12181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    12241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    12301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    12361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    12421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12481 atttccccga aaagtgccac ctgac
//
