LOCUS       sequence-434862-        2268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER retention signal (DGDL) for protein targeting to ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-869
FEATURES             Location/Qualifiers
     source          1..2268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        177..281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             503..1294
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1465..2053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1954..1973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2207..2224
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggccgatt cattaatcac tctgtggtct cattcggacg gcgacctgta ggctttgaga
       61 ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta
      121 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt
      301 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
      361 atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
      421 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
      481 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
      541 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
      601 gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac
      661 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
      721 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
      781 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
      841 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
      901 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
      961 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     1021 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     1081 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     1141 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     1201 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt
     1261 aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat
     1321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcg
//
