LOCUS       sequence-434864-        8950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9, LuxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
  TITLE     Multistable and dynamic CRISPRi-based synthetic circuits.
  JOURNAL   Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1.
  PUBMED    32488086
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-2"; volume: "11(1):2746. doi"; pages: " 
            10.1038/s41467-020-16574-1"
COMMENT     Sequence Label: pJ2018
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..179
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     163..179
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        213..246
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     RBS             317..328
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(335..361,362..364,365..2851,2852..2854,2855..4441)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        4774..4808
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        4774..4808
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        4774..4808
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             4878..4889
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        5957..5991
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        5957..5991
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        5957..5991
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             6061..6072
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6079..6642
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     primer_bind     complement(6812..6830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6826..6873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6882..6968
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7060..7087
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7216..8007)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(8008..8099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgctc ctaggatgca gtgactcgag ttgtaaaacg acggccagtc
      181 aattggaatt cgagctccag cctgcggtcc ggttgacagc tagctcagtc ctaggtattg
      241 tactagttcg ctgggacgcc cgagtgacga ctgcgaagta acctctattt atcagcctgc
      301 ggtccggggt accattaaag aggagaaaga attcatggat aagaaatact caataggctt
      361 agctatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc
      421 taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg
      481 ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg
      541 tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga
      601 gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga
      661 agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca
      721 tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc
      781 ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt
      841 gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt
      901 acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa
      961 agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct
     1021 ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac
     1081 ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga
     1141 tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt
     1201 gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa
     1261 tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca
     1321 tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga
     1381 aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca
     1441 agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt
     1501 attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc
     1561 tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt
     1621 ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc
     1681 ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc
     1741 tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca
     1801 atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc
     1861 aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata
     1921 tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt
     1981 tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt
     2041 caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc
     2101 ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa
     2161 tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag
     2221 ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa
     2281 acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg
     2341 tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc
     2401 caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca
     2461 aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg
     2521 tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa
     2581 agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac
     2641 aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa
     2701 agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga
     2761 aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga
     2821 tattaatcgt ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga
     2881 cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa
     2941 cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc
     3001 caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag
     3061 tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa
     3121 gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact
     3181 tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga
     3241 tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct
     3301 aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt
     3361 ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat
     3421 aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga
     3481 aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac
     3541 tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat
     3601 gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc
     3661 aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa
     3721 aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt
     3781 ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat
     3841 ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga
     3901 agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg
     3961 tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc
     4021 aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc
     4081 agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat
     4141 tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa
     4201 agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat
     4261 tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac
     4321 aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca
     4381 tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacta
     4441 atcgctggga cgcccgccat ggttcagcca aaaaacttaa gaccgccggt cttgtccact
     4501 accttgcagt aatgcggtgg acaggatcgg cggttttctt ttctcttctc aattcttctg
     4561 acctgtaacg aataatagat agtaaagtag tctccgattg agttttctct gccgagtccc
     4621 acccagttct gtgatttcag taagttggta attgatacac tgttgcgaga actgctgcct
     4681 ggtagtagat aggttgttat tgagtaagaa ggtaaagtga acgaaatccc tgaaactgag
     4741 actgtagaaa ataagcttca gcctgcggtc cggtttacgg ctagctcagt cctaggtatt
     4801 atgctagctc gctgggacgc ccggggacta cacttacgaa actattgatt gctcagcctg
     4861 cggtccgggg taccattaaa gaggagaaag aattcatgaa aaacatcaac gccgacgata
     4921 cgtaccgcat catcaacaaa atcaaagctt gtcgctcaaa caacgacatc aaccagtgcc
     4981 tgagcgatat gaccaaaatg gttcattgcg aatattacct gctggcgatt atctatccgc
     5041 acagtatggt caaatcagat atttcgatcc tggacaacta cccgaaaaaa tggcgtcagt
     5101 attacgatga cgccaatctg attaaatatg atccgatcgt ggactacagt aactccaatc
     5161 actccccgat taactggaat atctttgaaa acaatgcggt caacaaaaaa tctccgaacg
     5221 tgatcaaaga agccaaaacc agcggcctga ttacgggttt ttcgttcccg atccataccg
     5281 caaacaatgg ctttggtatg ctgagcttcg ctcactctga aaaagataac tatattgaca
     5341 gcctgttcct gcatgcatgc atgaatattc cgctgatcgt gccgtctctg gttgataact
     5401 accgtaaaat caacatcgct aacaacaaaa gtaacaatga cctgaccaaa cgcgaaaaag
     5461 aatgcctggc gtgggcctgt gaaggcaaaa gctcttggga tattagtaaa atcctgggtt
     5521 gttccgaacg taccgttacg tttcatctga cgaacgcgca aatgaaactg aacaccacga
     5581 atcgctgcca atccatctcc aaagcaatcc tgaccggcgc tatcgactgt ccgtacttca
     5641 aaaattaata atcgctggga cgcccgcctg caggctcggt accaaattcc agaaaagagg
     5701 cctcccgaaa ggggggcctt ttttcgtttt ggtcctactg attgggtgaa ctacgatact
     5761 tccagaataa cgagaaatac gctactctta cagataaaac tcactgccga ttacggatag
     5821 gacgaacaac actttgatta tcttacgatt taccgaaggt tctattgagt tggacccagc
     5881 agttactact tttattcagt gattctgtct tatcgggaga ttgttcgctt accacgtcga
     5941 ccagcctgcg gtccggttta cggctagctc agtcctaggt attatgctag ctcgctggga
     6001 cgcccgagat agccgttaca caggtgacac ttatttcagc ctgcggtccg gggtaccatt
     6061 aaagaggaga aagaattcat ggaccactac ctcgacattc gcttgcgacc ggacccggaa
     6121 tttcccccgg cgcaactcat gagcgtgctc ttcggcaagc tccaccaggc cctggtggca
     6181 cagggcgggg acaggatcgg cgtgagcttc cccgacctcg acgaaagccg ctcccggctg
     6241 ggcgagcgcc tgcgcattca tgcctcggcg gacgaccttc gtgccctgct cgcccggccc
     6301 tggctggaag ggttgcggga ccatctgcaa ttcggagaac cggcagtcgt gcctcacccc
     6361 acaccgtacc gtcaggtcag tcgggttcag gcgaaaagca atccggaacg cctgcggcgg
     6421 cggctcatgc gccggcacga tctgagtgag gaggaggctc ggaaacgcat tcccgatacg
     6481 gtcgcgagag ccttggacct gcccttcgtc acgctacgca gccagagcac cggacagcac
     6541 ttccgtctct tcatccgcca cgggccgttg caggtgacgg cagaggaagg aggattcacc
     6601 tgttacgggt tgagcaaagg aggtttcgtt ccctggttct gataatcgct gggacgcccg
     6661 gcggccgcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
     6721 aaaggtagcg aacgacgagt cactgttgag gataaatact ttctctacta ggcgcctgtt
     6781 acacaggtcc tcagcggcgc gcctgctgcc accgctgagc aataactagc ataacccctt
     6841 ggggcctcta aacgggtctt gaggggtttt ttgccaggca tcaaataaaa cgaaaggctc
     6901 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     6961 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg
     7021 caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg
     7081 gcctttttgc gtttcagatc taccggtaaa ccagcaatag acataagcgg ctatttaacg
     7141 accctgccct gaaccgacga ccgggtcatc gtggccggat cttgcggccc ctcggcttga
     7201 acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     7261 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     7321 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     7381 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     7441 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     7501 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     7561 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     7621 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     7681 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     7741 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     7801 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     7861 atcaatatca ctgtgtggct tcaggccgtc atccactgcg gagccgtaca aatgtacggc
     7921 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     7981 tacttcggcg atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta
     8041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8101 agctagctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca
     8161 tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacagc
     8221 agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac
     8281 agacgctttt ccggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg
     8341 ggcgaaaccc gacaggactt aaagatcccc accgtttccg gcgggtcgct ccctcttgcg
     8401 ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc cgcctttctc ccttacggga
     8461 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgtttgct
     8521 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
     8581 actgttcact tgagtccaac ccggaaaagc acggtaaaac gccactggca gcagccattg
     8641 gtaactggga gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc
     8701 gccaaagtcc ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt
     8761 accacggtta agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg
     8821 ttttttcgaa ggttaagtca gttggggaac tgcttaacct tcgatcaaac cacctcccca
     8881 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     8941 tcctttgatc
//
