LOCUS       sequence-434882-        4861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CEBPA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Nakagawa S, Yamaguchi K, Takane K, Tabata S, Ikenoue T, Furukawa Y
  TITLE     Wnt/beta-catenin signaling regulates amino acid metabolism through 
            the suppression of CEBPA and FOXA1 in liver cancer cells.
  JOURNAL   Commun Biol. 2024 Apr 29;7(1):510. doi: 10.1038/s42003-024-06202-9.
  PUBMED    38684876
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-04-29"; volume: "7(1):510. doi"; pages: " 
            10.1038/s42003-024-06202-9"
COMMENT     Sequence Label: pCMV-Myc-CEBPA
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2000..2134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2024..2043)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2078..2097
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2245..2261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2245..2261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2258..2280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2269..2285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2293..2299,2300..2317,2318..2323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2338..2359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2476..2493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2647..3235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2727..2746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3406..4197,4198..4266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4029..4048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4267..4371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4439..4457
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4495..4517)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4617..4636
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4830..4852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4844..4861
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4845..4861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 cagaagctga tctcagagga ggacctgctt atggccatgg aggcccgaat tcccatggag
      901 tcggccgact tctacgaggc ggagccgcgg cccccgatga gcagccacct gcagagcccc
      961 ccgcacgcgc ccagcagcgc cgccttcggc tttccccggg gcgcgggccc cgcgcagcct
     1021 cccgccccac ctgccgcccc ggagccgctg ggcggcatct gcgagcacga gacgtccatc
     1081 gacatcagcg cctacatcga cccggccgcc ttcaacgacg agttcctggc cgacctgttc
     1141 cagcacagcc ggcagcagga gaaggccaag gcggccgtgg gccccacggg cggcggcggc
     1201 ggcggcgact ttgactaccc gggcgcgccc gcgggccccg gcggcgccgt catgcccggg
     1261 ggagcgcacg ggcccccgcc cggctacggc tgcgcggccg ccggctacct ggacggcagg
     1321 ctggagcccc tgtacgagcg cgtcggggcg ccggcgctgc ggccgctggt gatcaagcag
     1381 gagccccgcg aggaggatga agccaagcag ctggcgctgg ccggcctctt cccttaccag
     1441 ccgccgccgc cgccgccgcc ctcgcacccg cacccgcacc cgcacccgcc gcccgcgcac
     1501 ctggccgccc cgcacctgca gttccagatc gcgcactgcg gccagaccac catgcacctg
     1561 cagcccggtc accccacgcc gccgcccacg cccgtgccca gcccgcaccc cgcgcccgcg
     1621 ctcggtgccg ccggcctgcc gggccctggc agcgcgctca aggggctggg cgccgcgcac
     1681 cccgacctcc gcgcgagtgg cggcagcggc gcgggcaagg ccaagaagtc ggtggacaag
     1741 aacagcaacg agtaccgggt gcggcgcgag cgcaacaaca tcgcggtgcg caagagccgc
     1801 gacaaggcca agcagcgcaa cgtggagacg cagcagaagg tgctggagct gaccagtgac
     1861 aatgaccgcc tgcgcaagcg ggtggaacag ctgagccgcg aactggacac gctgcggggc
     1921 atcttccgcc agctgccaga gagctccttg gtcaaggcca tgggcaactg cgcgtgactc
     1981 gaggtaccgc ggccgcgggg atccagacat gataagatac attgatgagt ttggacaaac
     2041 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2101 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2161 gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt cgatctgcag
     2221 gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3061 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4621 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4681 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     4741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     4801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     4861 t
//
