LOCUS       sequence-434901-       13665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA 
            cloning cassette, facilitating high-fidelity cytosine base editing 
            in Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
  TITLE     Engineered cytosine base editor enabling broad-scope and 
            high-fidelity gene editing in Streptomyces.
  JOURNAL   Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
  PUBMED    38971862
REFERENCE   2  (bases 1 to 13665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-7"; volume: "15(1):5687. doi"; pages: " 
            10.1038/s41467-024-49987-3"
COMMENT     Sequence Label: pSCBE3-HF1
FEATURES             Location/Qualifiers
     source          1..13665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..804)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     primer_bind     complement(998..1018)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(998..1016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1045..1063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1236..1304,1305..2096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1454..1473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2756..2775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3009..3026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3240..3374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3264..3283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3318..3337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6592..6893
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     CDS             6894..7580
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             11742..11993
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     oriT            12356..12465
                     /note="incP origin of transfer"
     CDS             12498..12869
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_RNA        13025..13100
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag
       61 gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg
      121 gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag
      181 ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg
      241 taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc
      301 catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac
      361 ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg
      421 caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg
      481 ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg
      541 tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat
      601 gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc
      661 atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt
      721 aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac
      781 attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa
      841 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
      901 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
      961 acggcatcag ttaccgtgag ctgcaggtcg acggatccct ttagtgaggg ttaattgcgg
     1021 ccgcgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ctgacttccg cgtttccaga
     3061 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     3121 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     3181 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtcag
     3241 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3301 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3361 gcaataaaca agttcccgca cgatcagcgt cccgccgacc aggagcagtg cggccagcac
     3421 tgccgctaac gcctggtcct ggtcccggtc ctggtggtgc atcagtcctc cccgtgatca
     3481 cttcggcacc caccgtagtg atcacccccg acagcggatc aaggggtttg cgggtcccgg
     3541 tcggcgccgg gcgggggagg caggagccgc cgacgctgcc tctgggacgg gccggacggc
     3601 agggggaccg gcggccgggc gagctgcagc cgggggtccg gcagggccgg agcgggcgga
     3661 accgtgctct gacctgcggc ccgagtttcg tcacgtgacg gaatggaagg ctgctgcatt
     3721 tcgtcacgtg acgtatctcg gcgagcgact gccgacgcca cggcggacac gatcgcctcg
     3781 cgctggcgcc gggcctcgta cgcccgctgg cggcaggagc ggcggcagta gtcccggctc
     3841 cggccgacgc cggattgctt gatctccgag ccgcaccagg cgcagagctt cgcgccgtcg
     3901 gcgtccctgg gggtggtggt gctcatggcc gacgaccgta cgcggcacgt ctcgtagcga
     3961 ggcgagtcgg gcgcgaggta ccgcctgcac gaagtgccgg cggggccgac cccgggcgag
     4021 taatcccagg attactcccg cggcttcgac cccggccgcc gtcgccgcgt acgtcaccga
     4081 cccccgccgt acgtcaccgg gatgacgtac ggcggggggg agcgagttag tgcgaagtgg
     4141 gcccacttgc gagccgggcg atgtgccggg cggcccgctc ctggcggtcg tcggcgtcgt
     4201 cgtcctggtc gtcgtcctgc tctcgccgtc ggcgtgcagt tgcttcctcg cggcgctggg
     4261 cgagggcggc gagcatgtcg gcgtacgcct cggccacctc ccccgccgtg agcaccacca
     4321 ctgtgtcggc cgcgtcggcc agcgccagga cctcccgcac ccgttcgccc acggccgccg
     4381 aatcctcgtt gccgtccttg ccttcggcgg cccgggtcgc ctcgaggtcg agggcgcggc
     4441 gggtgaccgc gtgccatccg tcctcggtca cggcgacccc ggcccgcagc tccccgccgt
     4501 cggcgtcggc cgccaggagc agatcgaggt cgtcggcctc ggtgtcgccg ccgtcgagcc
     4561 cgagcatctg ccgcaggtag cgggtccatt cgatggcccg gcgtccccgg gttgcccgct
     4621 cgtactcgtg ccagcgcgag aggttccact ccagcgagcc gaccccggcg gcgtcgtcct
     4681 cggtcatgcc gccggtcagg tccccgatcc gtccgaggag ttcgaacggg gcgacgttcc
     4741 cgccggtcgc cgtcttgagg tcggcgcggg cgagttcgag ggcgggcgcc ttcccgtcct
     4801 gggtcttggc gatgtactcg gcgaggtcgt tggcgtcgcg ctcggtctcc agccgcttga
     4861 agtcgacgcc gtgccggtcg tcgggcgtga aggcggggtt gaccttgcgc agggcggcgg
     4921 tccacacgga ccgccagtgc ccctgccact cgtcgagcgc ggcgccggtc ggctcgaagg
     4981 tggcgacgat ctgcttcgcg gaccgctccc cctcggtccg gccgccgacc aggacgatcg
     5041 cgtggatgtg cgggtgccag ccgttgatct gccccacggt gacttcggtc gcgcggatca
     5101 tgccgacgta cccgatccgg tctcggatgc cctcgcggtc ggcggcccgg tgcccgtcct
     5161 tggcccggcg tccggcccac gtgccgcccg tgatcagtcg ctggtaggcg cccggccgcc
     5221 gggggctgtc cggcgtcttc cgggtgccct ggagggcgtc catgaggtcc gcgagccggt
     5281 ccgtgtgccc atggcgggcc gtgaaggtga ccaggtaggc ggtccccccg cgcttgatcc
     5341 actcgaccac ggcggcggtg atctcctcgg cccgcttgtg ccggatcgtg gcggcgcaga
     5401 ccgggcagag ccagatccgc ccgcaccgca tcaggcccag gaccacggac gttccggccg
     5461 ccgtctgggc gacgatcacg ccggaggcag ggtccatcag ggcgcggccg cagcccttgc
     5521 acgcggcgtc cccgctgatc cgccacagcg tccggcggcg gctgtaccgg gcggctttcc
     5581 gcagtcgggc agcgtcgctc cgcgacgtgc ttcctacttc cgagaggctg tcgcctctcg
     5641 ggctctcccc atccaccccg tccggagaaa ccgcaggtcg gaggggtgcg ggaaactctg
     5701 ttgtttcttt cccaaggtgt tcgcttttgc ctcgggcggc atctcgcgtc acacgcgcga
     5761 tcgcccgctt cgctgccatc cggcagcggt ctgagcagta gatacgcggc cgtttgcccg
     5821 gtgtgtgggc aattgcggtc ccgcagtggc agcggggccc ggcgggccga tctggcaatg
     5881 cctcggcatc gctccgtact ctgggcacga gcaacgttcc tgtctcgccc ggctaagggg
     5941 cgcgagtctg ggagcggacg ggtcggaggt gcgaagtccg gcccgttgct ctttggtctg
     6001 gtgggaatcc tggcaccaat cgggccagag gttccctccg ccactcccga cgccccttgg
     6061 ggctggtgtg acttggaggg ccgaagagag ccccgccggt gatccggcgg ggctttgacg
     6121 tgcggtcagt gcgtgtgtcg gcgagcgatg gccacgaggc cctggaagcc gagcggtccg
     6181 gcgaagtcgg cccagtcgca accgggctca gcgcagtggg cggaccagcc accgccgttg
     6241 gggtcctgga ccaggttcac ggtcccctcg gtcaggcgtc cgtcgaagtc ggtcatggtc
     6301 ggtctcctgg tgggtggggg cggggcgcca gcacgaagtg ccggcgcccc gcgggggttg
     6361 gtcgggtcag gcgccgaacc ggcgggcggc ggcggcgacc aggccgtcgg cggcggccat
     6421 ggcgcggtcg cggtcggtgg tgagggcggt gcggtcggcg gcggcccagt cctgctctca
     6481 cgcaacgtct acgtggacgc tcagggcgac acgatcgagg tcggggagtc cgtcgctccg
     6541 gcgggccgct ggcgcgtcca ccgggactga tcaaggcgaa tacttcatat ggccctgcag
     6601 gcggaagtca ggtagacacg acttccgcta gtccttgcaa ggtctgctga cgtgaggcgg
     6661 ggcggtcgtt tttgaccgcc ctgccttcgt catgtaggct cgctcgctgt gcctggcgtg
     6721 tcatcagacg cccaggtccc ggtgccgtga ggcccgggcc atcgagccgg tggtacgtgg
     6781 ctgcggtccc cttgtgaggg ctgcgcgccg tgtgctgtcc ggcgcgcaca gccttgaatc
     6841 cacccgcggg ggccggccgg tctccgtgag ctcgagtaga cgacggagac gtaatgtcgt
     6901 ccgagaccgg cccggtggcc gtggacccga ccctgcgtcg ccgcatcgag ccgcacgagt
     6961 tcgaggtgtt cttcgacccg cgggagctgc gcaaggagac ctgcctgctg tacgagatca
     7021 actggggcgg ccggcactcg atctggcgcc acacctcgca gaacaccaac aagcacgtcg
     7081 aggtgaactt catcgagaag ttcaccaccg agcggtactt ctgcccgaac acccgctgct
     7141 cgatcacctg gttcctgtcc tggtccccgt gcggcgagtg ctcccgggcg atcaccgagt
     7201 tcctgtcccg ctacccgcac gtcaccctgt tcatctacat cgcccggctg taccaccacg
     7261 ccgacccgcg gaaccggcag ggcctgcgcg acctgatctc ctccggcgtg accatccaga
     7321 tcatgaccga gcaggagtcc ggctactgct ggcggaactt cgtcaactac tcgccgtcca
     7381 acgaggccca ctggccgcgg tacccgcacc tgtgggtgcg cctgtacgtc ctggagctgt
     7441 actgcatcat cctgggcctg ccgccgtgcc tgaacatcct gcgtcgcaag cagccgcagc
     7501 tgaccttctt caccatcgcc ctgcagtcct gccactacca gcgcctgccg ccgcacatcc
     7561 tgtgggcgac cggcctgaag tccggctcgg agaccccggg cacctcggag tccgccaccc
     7621 cggagtccga caagaagtac agcatcggcc tggccatcgg caccaacagc gtgggctggg
     7681 cggtcatcac cgacgagtac aaggtcccct ccaagaagtt caaggtcctg ggcaacaccg
     7741 accggcactc gatcaagaag aacctgatcg gcgccctgct cttcgacagc ggcgagaccg
     7801 ccgaggcgac ccgcctgaag cggaccgcgc gccggcgcta cacccggcgc aagaaccgca
     7861 tctgctacct ccaggaaatc ttctccaacg agatggccaa ggtcgacgac tcgttcttcc
     7921 accggctcga ggagagcttc ctggtggagg aggacaagaa gcacgagcgc cacccgatct
     7981 tcggcaacat cgtcgacgag gtggcctacc acgagaagta ccccaccatc taccacctcc
     8041 gcaagaagct ggtcgactcg accgacaagg cggacctgcg gctcatctac ctggccctcg
     8101 cgcacatgat caagttccgc ggccacttcc tcatcgaggg cgacctgaac ccggacaact
     8161 ccgacgtcga caagctcttc atccagctgg tgcagaccta caaccagctg ttcgaggaga
     8221 accccatcaa cgccagcggc gtcgacgcca aggcgatcct ctccgcgcgc ctgagcaagt
     8281 cccggcgcct ggagaacctc atcgcccagc tgccgggcga gaagaagaac ggcctcttcg
     8341 gcaacctgat cgcgctgtcg ctcggcctga cccccaactt caagagcaac ttcgacctgg
     8401 ccgaggacgc gaagctccag ctgtccaagg acacctacga cgacgacctg gacaacctgc
     8461 tcgcccagat cggcgaccag tacgcggacc tcttcctggc cgcgaagaac ctctcggacg
     8521 ccatcctgct cagcgacatc ctgcgggtca acaccgagat caccaaggcc ccgctgtcgg
     8581 cgagcatgat caagcggtac gacgagcacc accaggacct gaccctgctc aaggccctcg
     8641 tgcgccagca gctgcccgag aagtacaagg aaatcttctt cgaccagtcc aagaacggct
     8701 acgccggcta catcgacggc ggcgcgtcgc aggaggagtt ctacaagttc atcaagccga
     8761 tcctggagaa gatggacggc accgaggagc tgctcgtcaa gctgaaccgc gaggacctgc
     8821 tccgcaagca gcggaccttc gacaacggct ccatcccgca ccagatccac ctgggcgagc
     8881 tgcacgccat cctccggcgc caggaggact tctacccctt cctgaaggac aaccgcgaga
     8941 agatcgagaa gatcctgacc ttccgcatcc cgtactacgt cggccccctg gcccgcggca
     9001 actcccggtt cgcgtggatg acccggaagt cggaggagac catcaccccg tggaacttcg
     9061 aggaggtcgt ggacaagggc gcctccgccc agtcgttcat cgagcgcatg accgccttcg
     9121 acaagaacct cccgaacgag aaggtcctgc ccaagcactc cctgctctac gagtacttca
     9181 ccgtgtacaa cgagctgacc aaggtcaagt acgtgaccga gggcatgcgg aagccggcct
     9241 tcctgtcggg cgagcagaag aaggcgatcg tcgacctgct cttcaagacc aaccgcaagg
     9301 tcaccgtgaa gcagctgaag gaggactact tcaagaagat cgagtgcttc gactccgtcg
     9361 agatcagcgg cgtggaggac cgcttcaacg cctccctggg cacctaccac gacctgctca
     9421 agatcatcaa ggacaaggac ttcctcgaca acgaggagaa cgaggacatc ctggaggaca
     9481 tcgtcctcac cctgaccctc ttcgaggacc gcgagatgat cgaggagcgg ctcaagacct
     9541 acgcccacct gttcgacgac aaggtgatga agcagctgaa gcggcgccgg tacaccggct
     9601 ggggcgccct ctcccggaag ctgatcaacg gcatccggga caagcagagc ggcaagacca
     9661 tcctggactt cctcaagtcc gacggcttcg ccaaccgcaa cttcatggcg ctcatccacg
     9721 acgacagcct gaccttcaag gaggacatcc agaaggccca ggtctcgggc cagggcgaca
     9781 gcctccacga gcacatcgcc aacctggcgg gctccccggc gatcaagaag ggcatcctcc
     9841 agaccgtcaa ggtcgtggac gagctggtca aggtgatggg ccgccacaag cccgagaaca
     9901 tcgtgatcga gatggcccgg gagaaccaga ccacccagaa gggccagaag aactcgcgcg
     9961 agcggatgaa gcggatcgag gagggcatca aggagctggg cagccagatc ctgaaggagc
    10021 acccggtcga gaacacccag ctccagaacg agaagctgta cctctactac ctccagaacg
    10081 gccgcgacat gtacgtggac caggagctgg acatcaaccg gctgtccgac tacgacgtcg
    10141 accacatcgt gccgcagtcc ttcctgaagg acgactcgat cgacaacaag gtcctgaccc
    10201 gctcggacaa gaaccggggc aagtccgaca acgtgccctc ggaggaggtc gtgaagaaga
    10261 tgaagaacta ctggcgccag ctgctcaacg ccaagctcat cacccagcgc aagttcgaca
    10321 acctgaccaa ggccgagcgg ggcggcctga gcgagctgga caaggcgggc ttcatcaagc
    10381 gccagctggt cgagacccgg gcgatcacca agcacgtggc ccagatcctg gactcccgga
    10441 tgaacaccaa gtacgacgag aacgacaagc tgatccgcga ggtcaaggtg atcaccctca
    10501 agagcaagct ggtctccgac ttccgcaagg acttccagtt ctacaaggtc cgggagatca
    10561 acaactacca ccacgcccac gacgcgtacc tgaacgccgt cgtgggcacc gcgctgatca
    10621 agaagtaccc gaagctggag tccgagttcg tctacggcga ctacaaggtc tacgacgtgc
    10681 gcaagatgat cgccaagagc gagcaggaga tcggcaaggc caccgcgaag tacttcttct
    10741 actccaacat catgaacttc ttcaagaccg agatcaccct ggccaacggc gagatccgca
    10801 agcggcccct gatcgagacc aacggcgaga ccggcgagat cgtctgggac aagggccgcg
    10861 acttcgccac cgtccggaag gtgctgtcga tgccgcaggt caacatcgtg aagaagaccg
    10921 aggtgcagac cggcggcttc agcaaggagt ccatcctccc caagcgcaac agcgacaagc
    10981 tgatcgcccg gaagaaggac tgggacccga agaagtacgg cggcttcgac agccccaccg
    11041 tcgcctactc cgtgctggtc gtggcgaagg tcgagaaggg caagagcaag aagctgaagt
    11101 ccgtgaagga gctgctcggc atcaccatca tggagcgctc ctcgttcgag aagaacccga
    11161 tcgacttcct ggaggccaag ggctacaagg aggtcaagaa ggacctcatc atcaagctgc
    11221 ccaagtacag cctgttcgag ctggagaacg gccgcaagcg gatgctcgcc tccgcgggcg
    11281 agctgcaaaa gggcaacgag ctggccctcc cgtcgaagta cgtcaacttc ctgtacctcg
    11341 cgtcccacta cgagaagctg aagggctcgc ccgaggacaa cgagcagaag cagctcttcg
    11401 tggagcagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttcagcaagc
    11461 gcgtcatcct ggccgacgcg aacctcgaca aggtgctgtc cgcctacaac aagcaccgcg
    11521 acaagccgat ccgggagcag gcggagaaca tcatccacct gttcaccctc accaacctgg
    11581 gtgccccggc cgccttcaag tacttcgaca ccaccatcga ccgcaagcgg tacacctcca
    11641 ccaaggaggt cctcgacgcg accctgatcc accagagcat caccggcctg tacgagaccc
    11701 gcatcgacct gagccagctg ggcggcgact cgggcggctc caccaacctg tcggacatca
    11761 tcgagaagga gaccggcaag cagctggtga tccaggagtc catcctgatg ctgccggagg
    11821 aagtggagga agtgatcggc aacaagccgg agtcggacat cctggtgcac accgcctacg
    11881 acgagtcgac cgacgagaac gtcatgctgc tgacctcgga cgccccggag tacaagccgt
    11941 gggccctggt catccaggac tccaacggcg agaacaagat caagatgctg tgagaattca
    12001 gatctacgcg ttccccgcaa aagcggcctt tgactccctg caagcctcag cgaccgaata
    12061 tatcggttat gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct
    12121 gtttaagaaa ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg
    12181 gagccttttt ttaagcttgc atgcctgcag gtcgacggat cttttccgct gcataaccct
    12241 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
    12301 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
    12361 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
    12421 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa
    12481 cagatgaggg caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta
    12541 tcaaggtgta ctgccttcca gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg
    12601 gcatgagcct gtcggcctac ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg
    12661 tggactatga gcacgtccgc gagctggccc gcatcaatgg cgacctgggc cgcctgggcg
    12721 gcctgctgaa actctggctc accgacgacc cgcgcacggc gcggttcggt gatgccacga
    12781 tcctcgccct gctggcgaag atcgaagaga agcaggacga gcttggcaag gtcatgatgg
    12841 gcgtggtccg cccgagggca gagccatgac ttttttagcc gctaaaacgg ccggggggtg
    12901 cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga agagcgactt cgcggagctg
    12961 gtgaagtaca tcaccgacga gcaaggcaag accgatcccc ggggacctgc aggtcgactc
    13021 tagagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
    13081 aagtggcacc gagtcggtgc taaaaaaaaa ccccgcccct gacagggcgg ggtttttttt
    13141 gtagcctcag gagttgtagc gtgcacgcgg tcgatcttga cggctggcga gaggtgcggg
    13201 gaggatctga ccgacgcggt ccacacgtgg caccgcgatg ctgttgtggg cacaatcgtg
    13261 ccggttggta ggatccagct agctatttac ccgcaggaca tatccacgcc ctcctacatc
    13321 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
    13381 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
    13441 tcattgcccc cggacgagcg tctgctccgc cattcgccgt ccgccgtgcc aatcggatca
    13501 gccgtccaaa tgcgggattt tcgttagtcg gaggccaaac ggcattgagc gtcagcatat
    13561 catcagcgag ctgaagaaag acaatccccg atccgctcca cgtgttgccc cagcaatcag
    13621 cgcgaccttg cccctccaac gtcatctcgt tctccgctca tgagc
//
