LOCUS       sequence-434918-       10786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CRBN-BirA for CRBN substrate identification by E-STUB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Huang HT, Lumpkin RJ, Tsai RW, Su S, Zhao X, Xiong Y, Chen J, Mageed
            N, Donovan KA, Fischer ES, Sellers WR
  TITLE     Ubiquitin-specific proximity labeling for the identification of E3 
            ligase substrates.
  JOURNAL   Nat Chem Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9.
  PUBMED    38514884
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9."
COMMENT     Sequence Label: pLX307-CRBN-3F-BirA
FEATURES             Location/Qualifiers
     source          1..10786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1321..1330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2659..2724
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2728..3693
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             2728..3690
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     protein_bind    complement(3695..3719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3727..3768
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3819..4407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3872..3892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4290..4301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4479..4712
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4790..4911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4827..4846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4881..4900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4951..5086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5106..5125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5107..5125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5135..5152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5135..5151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5144..5166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5293..5748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5380..5399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5590..5611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5774..5878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5879..5947,5948..6739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6097..6116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6910..7498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7399..7418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7652..7669
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7786..7807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7822..7827,7828..7845,7846..7852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7860..7876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7865..7887
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7884..7900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7884..7900
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7919..7939
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7921..7939
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7967..8193
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8194..8374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8421..8546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9039..9272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9457..9501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9732..9942
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9754..9950
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9801..9936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9982..10584
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9982..10001)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10478..10498
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10627..10744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     1321 gccaccatgg ccggcgaagg agatcagcag gacgctgcgc acaacatggg caaccacctg
     1381 ccgctcctgc ctgcagagag tgaggaagaa gatgaaatgg aagttgaaga ccaggatagt
     1441 aaagaagcca aaaaaccaaa catcataaat tttgacacca gtctgccgac atcacataca
     1501 tacctaggtg ctgatatgga agaatttcat ggcaggactt tgcacgatga cgacagctgt
     1561 caggtgattc cagttcttcc acaagtgatg atgatcctga ttcccggaca gacattacct
     1621 cttcagcttt ttcaccctca agaagtcagt atggtgcgga atttaattca gaaagataga
     1681 acctttgctg ttcttgcata cagcaatgta caggaaaggg aagcacagtt tggaacaaca
     1741 gcagagatat atgcctatcg agaagaacag gattttggaa ttgagatagt gaaagtgaaa
     1801 gcaattggaa gacaaaggtt caaagtcctt gagctaagaa cacagtcaga tggaatccag
     1861 caagctaaag tgcaaattct tcccgaatgt gtgttgcctt caaccatgtc tgcagttcaa
     1921 ttagaatccc tcaataagtg ccagatattt ccttcaaaac ctgtctcaag agaagaccaa
     1981 tgttcatata aatggtggca gaaataccag aagagaaagt ttcattgtgc aaatctaact
     2041 tcatggcctc gctggctgta ttccttatat gatgctgaga ccttaatgga cagaatcaag
     2101 aaacagctac gtgaatggga tgaaaatcta aaagatgatt ctcttccttc aaatccaata
     2161 gatttttctt acagagtagc tgcttgtctt cctattgatg atgtattgag aattcagctc
     2221 cttaaaattg gcagtgctat ccagcgactt cgctgtgaat tagacattat gaataaatgt
     2281 acttcccttt gctgtaaaca atgtcaagaa acagaaataa caaccaaaaa tgaaatattc
     2341 agtttatcct tatgtgggcc gatggcagct tatgtgaatc ctcatggata tgtgcatgag
     2401 acacttactg tgtataaggc ttgcaacttg aatctgatag gccggccttc tacagaacac
     2461 agctggtttc ctgggtatgc ctggactgtt gcccagtgta agatctgtgc aagccatatt
     2521 ggatggaagt ttacggccac caaaaaagac atgtcacctc aaaaattttg gggcttaacg
     2581 cgatctgctc tgttgcccac gatcccagac actgaagatg aaataagtcc agacaaagta
     2641 atactttgct tgggttctga ctacaaagac catgacggtg attataaaga tcatgacatc
     2701 gattacaagg atgacgatga caagcttatg aaggataaca ccgtgccact gaaattgatt
     2761 gccctgttag cgaacggtga atttcactct ggcgagcagt tgggtgaaac gctgggaatg
     2821 agccgggcgg ctattaataa acacattcag acactgcgtg actggggcgt tgatgtcttt
     2881 accgttccgg gtaaaggata cagcctgcct gagcctatcc agttacttaa tgctaaacag
     2941 atattgggtc agctggatgg cggtagtgta gccgtgctgc cagtgattga ctccacgaat
     3001 cagtaccttc ttgatcgtat cggagagctt aaatcgggcg atgcttgcat tgcagaatac
     3061 cagcaggctg gccgtggtcg ccggggtcgg aaatggtttt cgccttttgg cgcaaactta
     3121 tatttgtcga tgttctggcg tctggaacaa ggcccggcgg cggcgattgg tttaagtctg
     3181 gttatcggta tcgtgatggc ggaagtatta cgcaagctgg gtgcagataa agttcgtgtt
     3241 aaatggccta atgacctcta tctgcaggat cgcaagctgg caggcattct ggtggagctg
     3301 actggcaaaa ctggcgatgc ggcgcaaata gtcattggag ccgggatcaa catggcaatg
     3361 cgccgtgttg aagagagtgt cgttaatcag gggtggatca cgctgcagga agcggggatc
     3421 aatctcgatc gtaatacgtt ggcggccatg ctaatacgtg aattacgtgc tgcgttggaa
     3481 ctcttcgaac aagaaggatt ggcaccttat ctgtcgcgct gggaaaagct ggataatttt
     3541 attaatcgcc cagtgaaact tatcattggt gataaagaaa tatttggcat ttcacgcgga
     3601 atagacaaac agggggcttt attacttgag caggatggaa taataaaacc ctggatgggc
     3661 ggtgaaatat ccctgcgtag tgcagaaaaa tgaaacccag ctttcttgta caaagtggtt
     3721 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     3781 agtaccggtt aagtcgacaa tcaacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     3841 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3901 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3961 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4021 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4081 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4141 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4201 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4261 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4321 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4381 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     4441 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4501 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4561 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4681 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     4741 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4921 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4981 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5041 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5101 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5161 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5221 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5281 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5341 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5401 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5461 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5521 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5581 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5641 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5701 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5761 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5821 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5881 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5941 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6001 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6061 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6121 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6181 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6241 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6301 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6361 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6421 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6481 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6541 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6601 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6661 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6721 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6781 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6841 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7021 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7561 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7621 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7681 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7741 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7801 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7861 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7921 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7981 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     8041 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     8101 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8161 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     8221 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8281 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8341 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8401 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8461 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8521 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8581 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8641 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8701 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8761 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8821 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8881 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8941 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9001 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9061 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9121 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9181 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9241 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9301 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9361 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9421 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9481 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9541 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9601 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9661 accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9721 caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag
     9781 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9841 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9901 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta
     9961 gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
    10021 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    10081 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    10141 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg
    10201 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    10261 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    10321 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    10381 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    10441 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    10501 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    10561 acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat
    10621 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    10681 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    10741 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccgg
//
