LOCUS       sequence-434924-       11201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Griffiths CD, Shah M, Shao W, Borgman CA, Janes KA
  TITLE     Three modes of viral adaption by the heart.
  JOURNAL   Sci Adv. 2024 Nov 15;10(46):eadp6303. doi: 10.1126/sciadv.adp6303. 
            Epub 2024 Nov 13.
  PUBMED    39536108
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-11-15"; volume: "10(46):eadp6303. doi"; pages: " 
            10.1126/sciadv.adp6303. Epub 2024 Nov 13"
COMMENT     Sequence Label: pEN_TT CVB3 A67G
FEATURES             Location/Qualifiers
     source          1..11201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..48
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    67..166
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        198..512
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    277..295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    349..367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    384..402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    420..438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     485..509
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(7971..8070)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8182..8201)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(8260..8279)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(8853..9386)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9575..9580,9581..9597,9598..9603))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      10260..10848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10749..10768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      11013..11099
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      join(11191..11201,1..17)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat
      181 tcagtcgact ggatcccgag tttactccct atcagtgata gagaacgtat gtcgagttta
      241 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
      301 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      361 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
      421 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
      481 gcagagctcg tttagtgaac cgtcagatcg ccactagtcg actggatccg gtaccgaatt
      541 cttaaaacag cctgtgggtt gatcccaccc acagggccca ttgggcgcta gcactctggt
      601 atcacggtac ctttgtgcgc ctgttttata ccccctcccc caactgtaac ttagaagtaa
      661 cacacaccga tcaacagtca gcgtggcaca ccagccacgt tttgatcaag cacttctgtt
      721 accccggact gagtatcaat agactgctca cgcggttgaa ggagaaagcg ttcgttatcc
      781 ggccaactac ttcgaaaaac ctagtaacac cgtggaagtt gcagagtgtt tcgctcagca
      841 ctaccccagt gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt
      901 ggctgcgttg gcggcctgcc catggggaaa cccatgggac gctctaatac agacatggtg
      961 cgaagagtct attgagctag ttggtagtcc tccggcccct gaatgcggct aatcctaact
     1021 gcggagcaca caccctcaag ccagagggca gtgtgtcgta acgggcaact ctgcagcgga
     1081 accgactact ttgggtgtcc gtgtttcatt ttattcctat actggctgct tatggtgaca
     1141 attgagagat cgttaccata tagctattgg attggccatc cggtgactaa tagagctatt
     1201 atatatccct ttgttgggtt tataccactt agcttgaaag aggttaaaac attacaattc
     1261 attgttaagt tgaatacagc aaaatgggag ctcaagtatc aacgcaaaag actggggcac
     1321 atgagaccag gctgaatgct agcggcaatt ccatcattca ctacacaaat attaattatt
     1381 acaaggatgc cgcatccaac tcagccaatc ggcaggattt cactcaagac ccgggcaagt
     1441 tcacagaacc agtgaaagat atcatgatta aatcactacc agggctcaac tcccccacag
     1501 tagaggagtg cggatacagt gacagggcga gatcaatcac attaggtaac tccaccataa
     1561 cgactcagga atgcgccaac gtggtggtgg gctatggagt atggccagat tatctaaagg
     1621 atagtgaggc aacagcagag gaccaaccga cccaaccaga cgttgccaca tgtaggttct
     1681 atacccttga ctctgtgcaa tggcagaaaa cctcaccagg atggtggtgg aagctgcccg
     1741 atgctttgtc gaacttagga ctgtttgggc agaacatgca gtaccactac ttaggccgaa
     1801 ctgggtatac cgtacatgtg cagtgcaatg catctaagtt ccaccaagga tgcttgctag
     1861 tagtgtgtgt accggaagct gagatgggtt gcgcaacgct agacaacacc ccatccagtg
     1921 cagaattgct ggggggcgat agcgcaaagg agtttgcgga caaaccggtc gcatccgggt
     1981 ccaacaagtt ggtacagagg gtggtgtata atgcaggcat gggggtgggt gttggaaacc
     2041 tcaccatttt cccccaccaa tggatcaacc tacgcaccaa taatagtgct acaattgtga
     2101 tgccatacac caacagtgta cctatggata acatgtttag gcataacaac gtcaccctaa
     2161 tggttatccc atttgtaccg ctagattact gccctgggtc caccacgtac gtcccaatta
     2221 cggtcacgat agccccaatg tgtgccgagt acaatgggtt acgtttagca gggcaccagg
     2281 gcttaccaac catgaatact ccggggagct gtcaatttct gacatcagac gacttccaat
     2341 caccatccgc catgccgcaa tatgacgtca caccagagat gaggatacct ggtgaggtga
     2401 aaaacttgat ggaaatagct gaggttgact cagttgtccc agtccaaaat gttggagaga
     2461 aggtcaactc tatggaagca taccagatac ctgtgagatc caacgaagga tctggaacgc
     2521 aagtattcgg ctttccactg caaccagggt actcgagtgt ttttagtcgg acgctcctag
     2581 gagagatctt gaactattat acacattggt caggcagcat aaagcttacg tttatgttct
     2641 gtggttcggc catggctact ggaaaattcc ttttggcata ctcaccacca ggtgctggag
     2701 ctcctacaaa aagggttgat gctatgcttg gtactcatgt aatttgggac gtggggctac
     2761 aatcaagttg cgtgctgtgt ataccctgga taagccaaac acactaccgg tttgttgctt
     2821 cagatgagta taccgcaggg ggttttatta cgtgctggta tcaaacaaac atagtggtcc
     2881 cagcggatgc ccaaagctcc tgttacatca tgtgtttcgt gtcagcatgc aatgacttct
     2941 ctgtcaggct attgaaggac actcctttca tttcgcagca aaactttttc cagggcccag
     3001 tggaagacgc gataacagcc gctataggga gagttgcgga taccgtgggt acagggccaa
     3061 ccaactcaga agctatacca gcactcactg ctgctgagac gggtcacacg tcacaagtag
     3121 tgccgggtga cactatgcag acacgccacg ttaagaacta ccattcaagg tccgagtcaa
     3181 ccatagagaa cttcctatgt aggtcagcat gcgtgtactt tacggagtat aaaaactcag
     3241 gtgccaagcg gtatgctgaa tgggtattaa caccacgaca agcagcacaa cttaggagaa
     3301 agctagaatt ctttacctac gtccggttcg acctggagct gacgtttgtc ataacaagta
     3361 ctcaacagcc ctcaaccaca cagaaccaag atgcacagat cctaacacac caaattatgt
     3421 atgtaccacc aggtggacct gtaccagata aagttgattc atacgtgtgg caaacatcta
     3481 cgaatcccag tgtgttttgg accgagggaa acgccccgcc gcgcatgtcc ataccgtttt
     3541 tgagcattgg caacgcctat tcaaatttct atgacggatg gtctgaattt tccaggaacg
     3601 gagtttacgg catcaacacg ctaaacaaca tgggcacgct atatgcaaga catgtcaacg
     3661 ctggaagcac gggtccaata aaaagcacca ttagaatcta cttcaaaccg aagcatgtca
     3721 aagcgtggat acctagacca cctagactct gccaatacga gaaggcaaag aacgtgaact
     3781 tccaacccag cggagttacc actactaggc aaagcatcac tacaatgaca aatacgggcg
     3841 catttggaca acaatcaggg gcagtgtatg tggggaacta cagggtggta aatagacatc
     3901 tagctaccag tgctgactgg caaaactgtg tgtgggaaag ttacaacaga gacctcttag
     3961 tgagcacgac cacagcacat ggatgtgata ttatagccag atgtcagtgc acaacgggag
     4021 tgtacttttg tgcgtccaaa aacaagcact acccaatttc gtttgaagga ccaggtctag
     4081 tagaggtcca agagagtgaa tactacccca ggagatacca atcccatgtg cttttagcag
     4141 ctggattttc cgaaccaggt gactgtggcg gtatcctaag gtgtgagcat ggtgtcattg
     4201 gcattgtgac catggggggt gaaggcgtgg tcggctttgc agacatccgt gatctcctgt
     4261 ggctggaaga tgatgcaatg gaacagggag tgaaggacta tgtggaacag cttggaaatg
     4321 cattcggctc cggctttact aaccaaatat gtgagcaagt caacctcctg aaagaatcac
     4381 tagtgggtca agactccatc ttagagaaat ctctaaaagc cttagttaag ataatatcag
     4441 ccttagtaat tgtggtgagg aaccacgatg acctgatcac tgtgactgcc acactagccc
     4501 ttatcggttg tacctcgtcc ccgtggcggt ggctcaaaca gaaggtgtca caatattacg
     4561 gaatccctat ggctgaacgc caaaacaata gctggcttaa gaaatttact gaaatgacaa
     4621 atgcttgcaa gggtatggaa tggatagctg tcaaaattca gaaattcatt gaatggctca
     4681 aagtaaaaat tttgccagag gtcagagaaa aacacgagtt cctgaacaga cttaaacaac
     4741 tccccttatt agaaagtcag atcgccacaa tcgagcagag cgcgccatcc caaagtgacc
     4801 aggaacaatt attttccaat gtccaatact ttgcccacta ttgcagaaag tacgctcccc
     4861 tctacgcagc tgaagcaaag agggtgttct cccttgagaa gaagatgagc aattacatac
     4921 agttcaagtc caaatgccgt attgaacctg tatgtttgct cctgcacggg agccctggtg
     4981 ccggcaagtc ggtggcaaca aacttaattg gaaggtcgct tgctgagaaa ctcaacagct
     5041 cagtgtactc actaccgcca gacccagatc acttcgacgg atacaaacag caggccgtgg
     5101 tgattatgga cgatctatgc cagaatcctg atgggaaaga cgtctccttg ttctgccaaa
     5161 tggtttccag tgtagatttt gtaccaccca tggctgccct agaagagaaa ggcattctgt
     5221 tcacctcacc gtttgtcttg gcatcgacca atgcaggatc tattaatgct ccaaccgtgt
     5281 cagatagcag agccttggca aggagatttc actttgacat gaacatcgag gttatttcca
     5341 tgtacagtca gaatggcaag ataaacatgc ccatgtcagt caagacttgt gacgatgagt
     5401 gttgcccggt caattttaaa aagtgctgcc ctcttgtgtg tgggaaggct atacaattca
     5461 ttgatagaag aacacaggtc agatactctc tagacatgct agtcaccgag atgtttaggg
     5521 agtacaatca tagacatagc gtggggacca cgcttgaggc actgttccag ggaccaccag
     5581 tatacagaga gatcaaaatt agcgttgcac cagagacacc accaccgccc gccattgcgg
     5641 acctgctcaa atcggtagac agtgaggctg tgagggagta ctgcaaagaa aaaggatggt
     5701 tggttcctga gatcaactcc accctccaaa ttgagaaaca tgtcagtcgg gctttcattt
     5761 gcttacaggc attgaccaca tttgtgtcag tggctggaat catatatata atatataagc
     5821 tctttgcggg ttttcaaggt gcttatacag gagtgcccaa ccagaagccc agagtgccta
     5881 ccctgaggca agcaaaagtg caaggccctg cctttgagtt cgccgtcgca atgatgaaaa
     5941 ggaactcaag cacggtgaaa actgaatatg gcgagtttac catgctgggc atctatgaca
     6001 ggtgggccgt tttgccacgc cacgccaaac ctgggccaac catcttgatg aatgatcaag
     6061 aggttggtgt gctagatgcc aaggagctag tagacaagga cggcaccaac ttagaactga
     6121 cactactcaa attgaaccgg aatgagaagt tcagagacat cagaggcttc ttagccaagg
     6181 aggaagtgga ggttaatgag gcagtgctag caattaacac cagcaagttt cccaacatgt
     6241 acattccagt aggacaggtc acagaatacg gcttcctaaa cctaggtggc acacccacca
     6301 agagaatgct tatgtacaac ttccccacaa gagcaggcca gtgtggtgga gtgctcatgt
     6361 ccaccggcaa ggtactgggt atccatgttg gtggaaatgg ccatcagggc ttctcagcag
     6421 cactcctcaa acactacttc aatgatgagc aaggtgaaat agaatttatt gagagctcaa
     6481 aggacgccgg gtttccagtc atcaacacac caagtaaaac aaagttggag cctagtgttt
     6541 tccaccaggt ctttgagggg aacaaagaac cagcagtact caggagtggg gatccacgtc
     6601 tcaaggccaa ttttgaagag gctatatttt ccaagtatat aggaaatgtc aacacacacg
     6661 tggatgagta catgctggaa gcagtggacc actacgcagg ccaactagcc accctagata
     6721 tcagcactga accaatgaaa ctggaggacg cagtgtacgg taccgagggt cttgaggcgc
     6781 ttgatctaac aacgagtgcc ggttacccat atgttgcact gggtatcaag aagagggaca
     6841 tcctctctaa gaagactaag gacctaacaa agttaaagga atgtatggac aagtatggcc
     6901 tgaacctacc aatggtgact tatgtaaaag atgagctcag gtccatagag aaggtagcga
     6961 aaggaaagtc taggctgatt gaggcgtcca gtttgaatga ttcagtggcg atgagacaga
     7021 catttggtaa tctgtacaaa actttccacc taaacccagg ggttgtgact ggtagtgctg
     7081 ttgggtgtga cccagacctc ttttggagca agataccagt gatgttagat ggacatctca
     7141 tagcatttga ttactctggg tacgatgcta gcttaagccc tgtctggttt gcttgcctaa
     7201 aaatgttact tgagaagctt ggatacacgc acaaagagac aaactacatt gactacttgt
     7261 gcaactccca tcacctgtac agggataaac attactttgt gaggggtggc atgccctcgg
     7321 gatgttctgg taccagtatt ttcaactcaa tgattaacaa tatcataatt aggacactaa
     7381 tgctaaaagt gtacaaaggg attgacttgg accaattcag gatgatcgca tatggtgatg
     7441 atgtgatcgc atcgtaccca tggcctatag atgcatcttt actcgctgaa gctggtaagg
     7501 gttacgggct gatcatgaca ccagcagata agggagagtg ctttaacgaa gttacctgga
     7561 ccaacgccac tttcctaaag aggtatttta gagcagatga acagtacccc ttcctggtgc
     7621 atcctgttat gcccatgaaa gacatacacg aatcaattag atggaccaag gatccaaaga
     7681 acacccaaga tcacgtgcgc tcactgtgtc tattagcttg gcataacggg gagcacgaat
     7741 atgaggagtt catccgtaaa attagaagcg tcccagtcgg acgttgtttg accctccccg
     7801 cgttttcaac tctacgcagg aagtggttgg actcctttta gattagagac aatttgaaat
     7861 aatttagatt ggcttaaccc tactgtgcta accgaaccag ataacggtac agtaggggta
     7921 aattctccgc attcggtgcg gcaattcgcg gccgcactcg agatatctag acccagcttt
     7981 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     8041 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
     8101 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     8161 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     8221 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     8281 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     8341 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     8401 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     8461 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     8521 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgtccctg cgccggttgc
     8581 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     8641 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     8701 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     8761 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattag
     8821 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     8881 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     8941 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
     9001 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     9061 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
     9121 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
     9181 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
     9241 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
     9301 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
     9361 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     9421 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
     9481 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     9541 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     9601 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     9661 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     9721 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     9781 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     9841 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     9901 ggactctagc tatagttcta gtggttggct actaataggt tgtattgatg ttggacgagt
     9961 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
    10021 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
    10081 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
    10141 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
    10201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10921 ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg ggacgtctaa
    10981 ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag
    11041 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
    11101 cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc
    11161 cgccataaac tgccaggcat caaactaagc agaaggccat c
//
