LOCUS       sequence-43499-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of a constitutively active form of H-Ras.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE puro H-Ras V12
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(45..77,78..80,81..614)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 cagtgtggtg gtacagcttg gggggccagg gccggggccg agcgatgacg gaatataagc
       61 tggtggtggt gggcgccgtc ggtgtgggca agagtgcgct gaccatccag ctgatccaga
      121 accattttgt ggacgaatac gaccccacta tagaggattc ctaccggaag caggtggtca
      181 ttgatgggga gacgtgcctg ttggacatcc tggataccgc cggccaggag gagtacagcg
      241 ccatgcggga ccagtacatg cgcaccgggg agggcttcct gtgtgtgttt gccatcaaca
      301 acaccaagtc ttttgaggac atccaccagt acagggagca gatcaaacgg gtgaaggact
      361 cggatgacgt gcccatggtg ctggtgggga acaagtgtga cctggctgca cgcactgtgg
      421 aatctcggca ggctcaggac ctcgcccgaa gctacggcat cccctacatc gagacctcgg
      481 ccaagacccg gcagggagtg gaggatgcct tctacacgtt ggtgcgtgag atccggcagc
      541 acaagctgcg gaagctgaac cctcctgatg agagtggccc cggctgcatg agctgcaagt
      601 gtgtgctctc ctgacgcagc acaagctcag gacatggagg tgccggatgc aggaaggagg
      661 tgcagacgga aggaggagga aggaaggacg gaagcaagga aggaaggaag ggctgctgga
      721 gcccagtcac cccccaagct gtaggaattc gccagcacag tggtcgaccc tgtggaatgt
      781 gt
//
