LOCUS       sequence-43503-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE8a-RFP
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(217..501)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(275..294)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cgacggcagc ggacaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga
       61 cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga
      121 actttgaaac gcatgaactc tttgataacg tcttcgctac tcgccatatg tatatctcct
      181 tcttaaaaga tcttttgaat tcccaaaaaa acgggtatgg agaaacagta gagagttgcg
      241 ataaaaagcg tcaggtagaa tccgctaatc ttatggataa aaatgctatg gcatagcaaa
      301 gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt
      361 tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg
      421 ttaccggttt ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc
      481 aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc
      541 aaaatgatcc cctgctgccg ggatactcgt ttaatgccca tctggtggcg ggtttaacgc
      601 cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
      661 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
      721 tttgccgacc gggtgatatt
//
