LOCUS       sequence-43505-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE8c-RFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      156..227
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      243..270
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(508..527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
       61 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
      121 taaggatcca aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
      181 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      241 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggcgttcgg ctgcggcgag
      301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      841 ggtggcctaa ctacggctac actagaagga cag
//
