LOCUS       sequence-43506-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE8c-RFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(214..498)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(272..291)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cggcagcgga cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga
       61 ccttcacctt caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact
      121 ttgaaacgca tgaactcttt gataacgtct tcgctactcg ccatatgtat atctccttct
      181 taaaagatct tttgaattcc caaaaaaacg ggtatggaga aacagtagag agttgcgata
      241 aaaagcgtca ggtagaatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg
      301 tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac
      361 gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta
      421 ccggtttggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat
      481 atggacaatt ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa
      541 atgatcccct gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga
      601 ttgaggccaa cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata
      661 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt
      721 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc
      781 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc
//
