LOCUS       sequence-43507-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE8k-RFP
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      158..229
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      245..272
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(510..529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
       61 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
      121 cttaaggatc caaactcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc
      181 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      241 cactggctca ccttcgggtg ggcctttctg cgtttatacc tagggcgttc ggctgcggcg
      301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      841 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
      901 gccagttacc ttcggaa
//
