LOCUS       sequence-435101-        4561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PU_Oryza sativa Ubiquitin3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_032
FEATURES             Location/Qualifiers
     source          1..4561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16..32)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(25..47)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        348..452
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1637..2225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2126..2145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2318..2335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(join(4547..4561,1..5))
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(join(4548..4561,1..5))
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
       61 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      121 ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct gaatggcgaa tgggagcgcc
      181 ctgtagcggc cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      241 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      301 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      361 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      421 aaatgcttca ataatattga aaaaggaaga gtatgcgtag ccgtaattgg agccgtaccc
      481 tgaccgaacg tagcggcggt aatggtgcgg tggcggtgtt tatggcgtgc tatgattgct
      541 tttttggcgt gcagagcatg ccgcgtgcga gcaaacagca ggcgcgttat gcggtgggcc
      601 gttgcctgat gctgtggagc agcaacgatg tgacccagca gggcagccgt ccgaaaacca
      661 aactgaacat tatgcgtgaa gcggtgattg cggaagtgag cacccagctg tctgaagtgg
      721 tgggcgtgat tgaacgtcat ctggaaccga ccctgctggc cgtgcatctg tatggcagcg
      781 cggtggatgg cggcctgaaa ccgcatagcg atattgatct gctggtgacc gtgaccgtgc
      841 gtctggatga aaccacccgt cgtgcgctga ttaacgatct gctggaaacc agcgcgagcc
      901 cgggtgaaag cgaaattctg cgtgcggtgg aagtgaccat cgtggtgcat gatgacatta
      961 ttccgtggcg ttatccggcg aaacgtgagc tgcagtttgg cgaatggcag cgtaacgata
     1021 ttctggccgg catttttgaa ccggcgacca ttgatatcga tctggccatt ctgctgacca
     1081 aagcgcgtga acatagcgtg gcgctggttg gtccggcagc ggaagaactg tttgatccgg
     1141 tgccggaaca ggacctgttt gaagcgctga acgaaaccct gaccctgtgg aacagcccgc
     1201 cggattgggc gggtgatgaa cgcaacgtgg tgctgaccct gagccgtatt tggtatagcg
     1261 cggtgaccgg caaaattgcg ccgaaagatg tggcggcgga ttgggccatg gaacgtctgc
     1321 cggcgcagta tcagccggtg attctggaag cgcgtcaggc gtatctgggc caggaagaag
     1381 atcgtctggc cagccgtgcg gatcagctgg aagaatttgt gcattacgtg aaaggcgaaa
     1441 ttaccaaagt ggtgggtaaa taataactgt cagaccaagt ttactcatat atactttaga
     1501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2281 attaggcacc ccaggcttta cccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2341 ggagagcgcc caatacgcaa ggaaacagct atgaccatgt taatgcagct ggcacgacag
     2401 gtttcccgac tggaaagcgg gcagtgaagt gaaggcccat gaggccagtt aattaagagg
     2461 tacccacttc gtggtctcac atttatctgg atctggaaga ggcaagaaag gattggaatt
     2521 aacataggta aaaaaaagca gctggagaat aagttggcac cagatcctac gatatcggta
     2581 ctgtcagctc aaaatagatg aacatatcaa tcacatcata ctacactaca attggacagc
     2641 atcattaatc ataagagcgc tgctcacagc tcacaccaca tcaacaagca acaaccaaaa
     2701 atatcttcag ataataacaa aactgacata agtaactaca ggacaacaga tctatgtaag
     2761 aacacagaat aaagccacct agttctagca tctgacagat ttgatttgca cgagtaaagc
     2821 aaataaaacg ggtaaaaaaa cacagtacaa catcagcact acagttcaac taggcaaaga
     2881 gccagataca atacagatta aattgacctt agaacccaag ctctacagtt caagtaggtg
     2941 tacacgatac agatgtaatt ctctaaccca gctccacaca attccaatcc aaatccaggg
     3001 gaaaaaaact aagaacacaa ttccaatcca aatccagagg aaataaacta tgtgtcgcct
     3061 aactcaacct aaagatttta acctaacgag ctattcatct attggaacaa tatcagaaca
     3121 atcccatccg aactaagcac aaataagtaa aactaaatct gatcacaaaa ctcaaactat
     3181 gtggcaggat caaaacataa ctgtgaacta ctcttattat ccatcattag ggtaatatat
     3241 cgaaacaatc cccatctgac acatcattag ggtaatatat cgaaacaatc cccatctgac
     3301 acatcattag ggtaatatat cgaaacaatc cccatctgac agaacaatct caaaaccaat
     3361 ccatctatta gatcgatata aataacacag atccctacct cgattctcac gaaaccctaa
     3421 atcaatgaga gattaagagg attaccctaa aatcgcacga aaccgtaact cgattaagag
     3481 gtggatcacc ctaaaatcgc acgaaaccct gagagattga gactaactcg cacgggatcc
     3541 tcctacgctt tccaccacga gcatcaaaat cgcgagatta gacgggcacc acgagagatt
     3601 cgaggattag gacgaggtga ttgattagtt accttgacga ggcgattaga gaacgcaaag
     3661 cttcgctccc cctccccttc ggtcgattcg actcgctatg cgattaatgg agagaaaacc
     3721 tctctatata aagcgtgggc aaggcggtgc gaggcagcat ccggtgcggt caccgccacg
     3781 cgtacggccc aatggcagca ccacacgtgt ccatgtagtt ggaggcgcga cacgtgtccg
     3841 gtcccggtac gaccatccag acggtgcccg gaaccaactc cgcgtcaggt catgccgcct
     3901 ctctttgacg aagtcggcat gcctgatcaa gaccgtacgt ttccgatcca acgatcagga
     3961 aacatcgtac ttgatctgct ataagctgcg tgtgggcctt aataatcctc tggggaaata
     4021 tttgtgggct gaaatgaacg ggatattggg cctagacaaa ccagggccca tgaattttac
     4081 ggcccaatag attgttgaca cctaattggc taagcccaac tcgcgcactc tgaccaaccg
     4141 atcggaagtt cgcgtccacg ccgccctgct cctccgtcgc cgccggctcg gcggctgacc
     4201 cccgcgccgc cgcaccagct gcgcgcgcgc gcgcgcgcgc ccccaccacc gcggcctctc
     4261 cctcccacgc gcagagggca cgggcagagg ccgccgcggc gccgtactcg ccggcgcttc
     4321 gtcggcctcg gcctcgggtc gaggccggtg gcggggcctc gtcagatcgc cccccttcct
     4381 gtgcagtaag tatttcccac ggacccaata tggattcgtt gttctaatcg atagctgcta
     4441 tcagagatgg gttcttctgc gtgatttacc tgttcttcac atcctcctga gaccacagag
     4501 tggagctcat ggcgcgccta ggccttgacg gccttcactc aattcgccct atagtgagtc
     4561 g
//
