LOCUS       sequence-435102-        3947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PU_Zea mays EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_033
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1508..1527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1509..1527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1538..1555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1538..1554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1547..1569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1870..1974
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3159..3747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3648..3667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3840..3857
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaagtgaagg cccatgaggc ccagcacttc gtggtctcac attggcaacg ggagcagctg
       61 actagaaagc tgtattcaca aaaaaaagga tatatatagc acgcagtcag gcaaaagtta
      121 agatatacta caattagcag ttgagaggtt ggtatttttt ttagaacagt tgcggcgatt
      181 cattgactaa catcaaatta gaaacacata tgtacgtaag cagactggta gattaattgt
      241 tcttttgttt agaatagcaa agacaacata gtcagtcatc aagaaaacag tgaccgtgca
      301 gtcagtgttc acccatctgt aactagtaat tcataccaat gaaaagcaac cgagcaagac
      361 aaatcagagc attcaacaca actgccgaat gaatacataa agatagatgc agcaggaaca
      421 aataatataa tatactaatt aaccaagcta tacaagatgc catgctaata aatataagta
      481 ggcaagcaaa catgatgtat ctaaaccaag aatacacaga cagatattgc ctagcaggct
      541 atgtgagtga gtctggcagt ggagcgaatt aaacaggaga tgccgaaact aaattgaagc
      601 acccaatagg attcgaacct atatcagaat tatatcaact acagtatacc acaaggcccg
      661 gtcccacggc ctcacccgag aggataggag atcagaccct ctgttttcac cgttagaaga
      721 tgggagcacc aattttagtt gaatttgaat ttaatcaggt gggcgatgcg atgatatata
      781 ctgtataact atttgatttg gtatatctgc tttattataa aagtggaaga aagaaaaacg
      841 attggtagct tagctaggtg cgcgacataa gagcaacact cctagtaata tatgcgtgaa
      901 ggacaaaaac tagggaacaa tttacagaac ttgaaagcct aataatgaat cgagcatcaa
      961 acaattcaga cagaactgaa tagtaacaac tgaatcaaaa ccgtgaattc aaggacccag
     1021 tgccttacct cgacggggta agtcaacaga cagagccggg ggaggggaga ggaggcggtc
     1081 gaggtgggac tgaacggtaa gggaggaatg gatggctctg tttatatagc gcgcgcctca
     1141 gcgccgcacg gtcaccggcc ggtccggcgg ctagggtttg cgagcgagta cagtcctcga
     1201 gctttcgtag cgcctacgaa cgccctgcct tgacggctac cctgccctag gcccactcca
     1261 ccaccgctgc ttcacgcgcc ggtcactcgt tctccgtgcc ttatcgtttt cttttctttc
     1321 gaaaaaacgg cgcttccttt cctttttctc atatcagaag aacaaaaaaa catgtgatga
     1381 aggatggcgc actttgctac gatgctagcg ggattctgct tgtcagaacc gatctctgtc
     1441 tggacttttc ggttgtggct cctgagacca cagagtgatg cggccttgac ggccttcact
     1501 caattcgccc tatagtgagt cgtattacgt cgcgctcact ggccgtcgtt ttacaacgtc
     1561 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     1621 ccagctggcg taatagcgaa gaggcccgca ccgaaacgcc cttcccaaca gttgcgcagc
     1681 ctgaatggcg aatgggagcg ccctgtagcg gccactcaac cctatctcgg tctattcttt
     1741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1801 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     1861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgt
     1981 agccgtaatt ggagccgtac cctgaccgaa cgtagcggcg gtaatggtgc ggtggcggtg
     2041 tttatggcgt gctatgattg cttttttggc gtgcagagca tgccgcgtgc gagcaaacag
     2101 caggcgcgtt atgcggtggg ccgttgcctg atgctgtgga gcagcaacga tgtgacccag
     2161 cagggcagcc gtccgaaaac caaactgaac attatgcgtg aagcggtgat tgcggaagtg
     2221 agcacccagc tgtctgaagt ggtgggcgtg attgaacgtc atctggaacc gaccctgctg
     2281 gccgtgcatc tgtatggcag cgcggtggat ggcggcctga aaccgcatag cgatattgat
     2341 ctgctggtga ccgtgaccgt gcgtctggat gaaaccaccc gtcgtgcgct gattaacgat
     2401 ctgctggaaa ccagcgcgag cccgggtgaa agcgaaattc tgcgtgcggt ggaagtgacc
     2461 atcgtggtgc atgatgacat tattccgtgg cgttatccgg cgaaacgtga gctgcagttt
     2521 ggcgaatggc agcgtaacga tattctggcc ggcatttttg aaccggcgac cattgatatc
     2581 gatctggcca ttctgctgac caaagcgcgt gaacatagcg tggcgctggt tggtccggca
     2641 gcggaagaac tgtttgatcc ggtgccggaa caggacctgt ttgaagcgct gaacgaaacc
     2701 ctgaccctgt ggaacagccc gccggattgg gcgggtgatg aacgcaacgt ggtgctgacc
     2761 ctgagccgta tttggtatag cgcggtgacc ggcaaaattg cgccgaaaga tgtggcggcg
     2821 gattgggcca tggaacgtct gccggcgcag tatcagccgg tgattctgga agcgcgtcag
     2881 gcgtatctgg gccaggaaga agatcgtctg gccagccgtg cggatcagct ggaagaattt
     2941 gtgcattacg tgaaaggcga aattaccaaa gtggtgggta aataataact gtcagaccaa
     3001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3781 gccttttgct ggccttttgc tcattaggca ccccaggctt tacccgaacg accgagcgca
     3841 gcgagtcagt gagcgaggaa gcggagagcg cccaatacgc aaggaaacag ctatgaccat
     3901 gttaatgcag ctggcacgac aggtttcccg actggaaagc gggcagt
//
