LOCUS       sequence-43512-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA8c-RFP
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..490)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(264..283)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac gaccttcacc
       61 ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa ctttgaaacg
      121 catgaactct ttgataacgt cttcgctact cgccatatgt atatctcctt cttaaaagat
      181 cttttgaatt cccaaaaaaa cgggtatgga gaaacagtag agagttgcga taaaaagcgt
      241 caggtagaat ccgctaatct tatggataaa aatgctatgg catagcaaag tgtgacgccg
      301 tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt acgcgttttt
      361 gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt taccggtttg
      421 gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca atatggacaa
      481 ttggtttctt ctctgaatgg cgggagtatg aaaagtatgg ctgaagcgca aaatgatccc
      541 ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc gattgaggcc
      601 aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat
      661 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ttgccgaccg
      721 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct
      781 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt
      841 a
//
