LOCUS       sequence-435131-       12973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Catalytically inactive dCasRx with 2A-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu
            Q, Zhang C
  TITLE     CRISPR-dCas13d-based deep screening of proximal and distal 
            splicing-regulatory elements.
  JOURNAL   Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
  PUBMED    38714659
REFERENCE   2  (bases 1 to 12973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-7"; volume: "15(1):3839. doi"; pages: " 
            10.1038/s41467-024-47140-8"
COMMENT     Sequence Label: dCas13d-BFP
FEATURES             Location/Qualifiers
     source          1..12973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    60..284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     273..290
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             316..496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1001..1017
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1002..1018
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1021..1609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1127..1138
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1536..1556
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1634..2332)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(2333..2386)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2402..2428)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2438..2458)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2468..5365)
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             complement(5375..5395)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5414..6592)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5423..6361
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5446..5466)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6717..6834
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7132..7176)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7361..7594
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8087..8212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8259..8439
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8457..8655)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8482..8502)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8657..9036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9208..9227
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9302..9406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9407..9475,9476..10267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9625..9644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10438..11026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10927..10946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11180..11197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11314..11335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11350..11355,11356..11373,11374..11380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11388..11404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11393..11415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11412..11428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11412..11428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    11477..11598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11488..11507)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11542..11561
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11728..12102)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(12121..12168)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(12216..12545)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12230..12365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12284..12303)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     12530..12550
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(join(12559..12973,1..14))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12669..12690)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12881..12900
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc
       61 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
      121 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
      181 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
      241 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
      301 ggtttaaacg ggccctgcta gagattttcc acactgacta aaagggtctg agggatctct
      361 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
      421 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
      481 ctaaccagag agacccagta cagtccggat gcagctctcg ggccatgtga tgaaatgcta
      541 ggcggctgtc aaacctccac tctaatactt ctctctccgg gtcatccatc ccatgcaggc
      601 tcacagggtg taacaagcgg gtgttctctc cttcattggc ttcttctacc ttctcttgct
      661 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgatc cctggccctg
      721 gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
      781 tatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
      841 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg
      901 gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg
      961 ctgtattgct acttgtgatt gctccatgtt tttctaggtc tcgaggtcga cggtatcgat
     1021 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     1081 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     1141 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     1201 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     1261 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     1321 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     1381 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     1441 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     1501 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     1561 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
     1621 tgatatcgaa ttcttaatta agcttgtgcc ccagtttgct agggaggtcg cagtatctgg
     1681 ccactgccac ctcgtgctgc tcgacgtagg tctcgttgtt ggcctccttg attctttcca
     1741 gtctgtagtc cacatagtag acgccaggca tcttgaggtt cttagcgggt ttcttggatc
     1801 tatatgtggt cttgatgttt gcgatcagat ggctcccgcc cacgagcttc agggccatgt
     1861 cgtttctgcc ttccaggccg ccgtcagcgg ggtacagcgt ctcggtgaag gcctcccagc
     1921 cgagtgtttt cttctgcatc acagggccgt tggatgtgaa gttcacccct ctgatcttga
     1981 cgttgtagat gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc
     2041 cgtcttcgta tgtggtgact ctctcccatg tgaagccctc agggaaggac tgcttgaaga
     2101 agtcggggat gccctgggtg tggttgatga aggtcttgct gccgtagagg aagctagtag
     2161 ccaggatgtc gaaggcgaag gggagagggc cgccctcgac caccttgatt ctcatggtct
     2221 gggtgccctc gtagggcttg ccttcgccct cggatgtgca cttgaagtga tggttgtcca
     2281 cggtgccctc catgtacagc ttcatgtgca tgttctcctt aatcagctcg cttgggccag
     2341 gattctcctc gacgtcaccg catgttagca gacttcctct gccctctcca ctgccgctag
     2401 cagcgtaatc tggaacatcg tatgggtaag cggccgccac cttcctcttt ttcttaggtc
     2461 cggatccgga attgccggac accttctttt tctccttgtc gaacttggcg gcctcgttcc
     2521 tatcgaacag ggcctcgatg ctcaggttct taaacctggg gatacagtag ccgaaaggca
     2581 cacacagcag tttcaggagc ctatcgttgt acttcttctc gtcattcaca gcgtcgaagt
     2641 actcggacac ctttccgctg cttttctcgt acctctcatt catgataatt ctctgcatga
     2701 tgtaatggta cagttggaag taggaattga cctcggcaat gtcgttgata taggcgtgga
     2761 catacctggc cacttccagg gcgacggcct tgtttgcgaa cagggtacat gtcttgttgt
     2821 caattctcag gaggtcctcc ctgatgatca cattccactt ggtgttcctc aggtaggcgt
     2881 tcagggcggt tttggccttc tccctgttaa tctgcctggt gaactcctcg gctttcttct
     2941 cgtcgctgta tttgatgtaa ttggcataca gcttggggtt ggcgctctcg aggctgtcaa
     3001 tgctctcctt ggctctttca gccatctcct tttccacgtc ctttctctta tcgggggcag
     3061 tttcatcaat gccagcgcag agcttggtga cggagctgaa tcccttctct tccagtttct
     3121 tgagattgat gtcgtagcct ttctccttgt acagttgagc atcacgctcg acgcaatgga
     3181 atccgatgac gtacctggcg ttgatattga caatattctt gaggatgtgg tagatcacgg
     3241 tgaggtacag gctgatgatc tttttaaact tctccctctc ggcgttttcc ctgccggtgt
     3301 cctcaatgac gctccttttc ttgtcgaatt ggtcgtagtt cattccggtg atgatctttg
     3361 tgagagcgtc caccttttcg ctcacgctct tgcccttatc ctttccgata caagtttcgt
     3421 agtacctgtc gatctggttc ttgccgttct ggccctgttt tttctggatg tcagcgatcc
     3481 tgccgagcac gaacttcacc acggcctcgt ttttggcgat ctcatggagg tgggcaggat
     3541 caccgtatct gatcaggtag tggaaccttt tattgctgat cacgttatta ataatgaaat
     3601 ttctcatgcc gtgcttgcct ttcttgagct tgtttccgtt ctcgtccagg gaaaaggtgt
     3661 cggcgagggc cttgagctca tcataggaca ggttggttcc taaaatacgg atggcgtcga
     3721 tatacatggc cctcctggca tcggcaatag gttctcccat tctagcgaag gacttgatca
     3781 gcctcagctc atcggcgatc ttggcggagt ctttgaaaaa ggcgtattcc tccacgaact
     3841 tagcgttgac tccgatgaga ggcatcacct tcaggaagct ctggatgtta tcgaatttat
     3901 taatcagggt ggtcaggagg tcgttgatct ccttgccatc caggaacatg gtcagggcat
     3961 acatgagttt gctgaaggcg gaaacatcac ggccagcggg caggattctg ggcagtctag
     4021 gggcgtcctt cttcttatac tctcttgtct tgtttcccct aaattccttg atgttgtgca
     4081 tgatattttc gagctttctc caaattctat tagcttcatc gtagtagagg gcatccttct
     4141 ggtcgtcgtt gaaggagccc ctcaggttaa tcacaaagat atccttctcg ctcagggact
     4201 tctcattatc ggggagggac ttattggcgg cagccacctt ggcatcctct tcgatgtaat
     4261 acctataaat cacaaagtcc atcatggtgt agaccttggt cctgatggag tcgaacacct
     4321 tatgattttt cctgatctcg gacatatcct tcctgtccag catcacttcc ctgagcttgg
     4381 tgatattgaa tccgaggttt ttctgctctt tcataatgct gaatctgaaa tattgttcgg
     4441 cgaattcggc agggttgatt cccagagttt cggcaatata gttcacgttg gcggcggagt
     4501 tcttggagaa ggagttggtc agctcattgg tgatcctgtc gtagaggtag ttgagggtgg
     4561 agatgtattc gttgtcgagg ttcttatcga ggttgtagag ccaggtcctg gagatcctgg
     4621 actcttcttc gttgttagcg accacccagt gcgccagtcc gctcaggagg gccagaatgt
     4681 catagcattc gttgccgtaa ttgatgatgt aatttctgcc ctccttgctg aaaaaggcct
     4741 ggccgaaata gccgagtctg gggttatcga ggaagttgtc gaactcgtca tactgggcct
     4801 tgatggcgtt gatgagctta tcgttattgt tgaaagcggc cctatggtgc tcggggtctt
     4861 tgaattcgtc gtaggtatac actgtggaga acttgccgaa tccaataatg tccttatcca
     4921 ggccggagat attgttgacg gcgtaggcgg cgttggtaat gtattcggcg aggatttttt
     4981 caatgtccag gatgttatgg atcacctgga tacaaatatt gtcattgcca tcagcgctct
     5041 cgccgaagta cctcttttcc agagtttcct tcaggccgag catatcctgc tggacgggtc
     5101 ctgtatacag agggttgtta gccaccacgg cgtagccctt aggatggctg aatttggcgt
     5161 tgccgatctt atagccggcg tttttatcgg ccatttcagc gctgaaggcc tcgccctcat
     5221 tcacgctcct gatgctgtcg ccctccacga tcttttccag cctggcgtcg ctgccttcgg
     5281 cgaaggttgt catgtacact ttggagccgg acacgagtgt ggacttcacg cccatgccct
     5341 tggcgaagga cttttttttt tcgatgctgg cctccacctt tctcttcttc ttggggctca
     5401 tggtggccgt acgtcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
     5461 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
     5521 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
     5581 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
     5641 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg
     5701 cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt
     5761 ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg
     5821 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca
     5881 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc
     5941 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa
     6001 aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa
     6061 aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga
     6121 gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct
     6181 caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac
     6241 ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag
     6301 gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta
     6361 cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
     6421 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
     6481 agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact
     6541 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag ccaattccca
     6601 ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta
     6661 tccatctttg cattaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     6721 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     6781 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     6841 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
     6901 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     6961 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     7021 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     7081 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     7141 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     7201 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     7261 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     7321 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     7381 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     7441 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     7501 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     7561 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     7621 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     7681 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     7741 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     7801 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     7861 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     7921 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     7981 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     8041 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     8101 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     8161 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     8221 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     8281 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     8341 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     8401 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     8461 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     8521 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     8581 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     8641 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     8701 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     8761 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     8821 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     8881 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     8941 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     9001 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     9061 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     9121 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     9181 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9241 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     9301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9421 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9481 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9541 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9601 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9661 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9721 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9781 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9841 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9901 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9961 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    10021 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    10081 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    10141 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    10201 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10261 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10321 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10561 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    11041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    11101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    11161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    11221 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    11281 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
    11461 cggtatacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    11521 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    11581 agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
    11641 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
    11701 ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca
    11761 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
    11821 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
    11881 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
    11941 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
    12001 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
    12061 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt
    12121 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca
    12181 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
    12241 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
    12301 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
    12361 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
    12421 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
    12481 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
    12541 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
    12601 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
    12661 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    12721 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    12781 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    12841 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    12901 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    12961 cgcttaatgc gcc
//
