LOCUS       sequence-435137-       10832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van 
            de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, 
            Jacobs T, Eyckerman S
  TITLE     Leveraging a self-cleaving peptide for tailored control in proximity
            labeling proteomics.
  JOURNAL   Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9.
  PUBMED    38986614
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2024-07-15"; volume: "4(7):100818. doi"; pages: " 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9"
COMMENT     Sequence Label: pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA >
            m...
FEATURES             Location/Qualifiers
     source          1..10832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..206
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      289..298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..342
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             349..1305
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1321..1374
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1384..3714
                     /codon_start=1
                     /gene="Homo sapiens NR3C1"
                     /product="human glucocorticoid receptor, isoform alpha"
                     /label=glucocorticoid receptor
                     /note="acts as a transcription factor that binds to 
                     glucocorticoid response elements, and as a modulator of 
                     other transcription factors"
                     /translation="MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASS
                     PSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGF
                     PQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDV
                     SSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDEN
                     CLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKE
                     DFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTAS
                     LSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMR
                     PDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCA
                     GRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGN
                     KTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVK
                     WAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMT
                     LPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYI
                     KELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEML
                     AEIITNQIPKYSNGNIKKLLFHQK"
     polyA_signal    3777..4001
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4020..4519
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4055..4078)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4452..4471
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4532..4930
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(4973..4992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5027..5046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5274..5336
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     6359..6375
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6359..6375
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6409..6425)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6410..6426)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6455..6475)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6455..6473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6494..6510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6494..6510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6507..6529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6518..6534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6542..6548,6549..6566,6567..6572))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6587..6608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6725..6742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6896..7484)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6976..6995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7655..8446,8447..8515))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8516..8620)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8646..9101)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8783..8804)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8995..9014
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9228..9250
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9242..9259
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9243..9259
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9269..9288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9269..9287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   10323..10357
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(10679..10697,10698..10720,10721..10739,10740..10762,
                     10763..10781,10782..10804,10805..10823,10824..10832,1..10,
                     11..29,30..52,53..71,72..94,95..113)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(10721..10739,10740..10762,10763..10781,10782..10804,
                     10805..10823,10824..10832,1..10,11..29,30..52,53..71,72..94,
                     95..113,114..136,137..155)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
       61 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      121 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgacc tatataagca
      181 gagctcgttt agtgaaccgt cagatcgcct gcagacgcca tccacgctgt tttgacctcc
      241 atagaagaca ccgggaccga tccagcctcc gcggccccga attgaacagc caccatgggc
      301 ggcaagccca tccccaaccc cctgctgggc ctggacagca ccgctagcaa agacaatact
      361 gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg
      421 ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac
      481 tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg
      541 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
      601 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
      661 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
      721 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
      781 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
      841 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
      901 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
      961 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     1021 caggaagcag ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg
     1081 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag
     1141 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     1201 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     1261 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagggagg tggctccgga
     1321 gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccaggaggc
     1381 tccatggact ccaaagaatc attaactcct ggtagagaag aaaaccccag cagtgtgctt
     1441 gctcaggaga ggggagatgt gatggacttc tataaaaccc taagaggagg agctactgtg
     1501 aaggtttctg cgtcttcacc ctcactggct gtcgcttctc aatcagactc caagcagcga
     1561 agacttttgg ttgattttcc aaaaggctca gtaagcaatg cgcagcagcc agatctgtcc
     1621 aaagcagttt cactctcaat gggactgtat atgggagaga cagaaacaaa agtgatggga
     1681 aatgacctgg gattcccaca gcagggccaa atcagccttt cctcggggga aacagactta
     1741 aagcttttgg aagaaagcat tgcaaacctc aataggtcga ccagtgttcc agagaacccc
     1801 aagagttcag catccactgc tgtgtctgct gcccccacag agaaggagtt tccaaaaact
     1861 cactctgatg tatcttcaga acagcaacat ttgaagggcc agactggcac caacggtggc
     1921 aatgtgaaat tgtataccac agaccaaagc acctttgaca ttttgcagga tttggagttt
     1981 tcttctgggt ccccaggtaa agaaacgaat gagagtcctt ggagatcaga cctgttgata
     2041 gatgaaaact gtttgctttc tcctctggcg ggagaagacg attcattcct tttggaagga
     2101 aactcgaatg aggactgcaa gcctctcatt ttaccggaca ctaaacccaa aattaaggat
     2161 aatggagatc tggttttgtc aagccccagt aatgtaacac tgccccaagt gaaaacagaa
     2221 aaagaagatt tcatcgaact ctgcacccct ggggtaatta agcaagagaa actgggcaca
     2281 gtttactgtc aggcaagctt tcctggagca aatataattg gtaataaaat gtctgccatt
     2341 tctgttcatg gtgtgagtac ctctggagga cagatgtacc actatgacat gaatacagca
     2401 tccctttctc aacagcagga tcagaagcct atttttaatg tcattccacc aattcccgtt
     2461 ggttccgaaa attggaatag gtgccaagga tctggagatg acaacttgac ttctctgggg
     2521 actctgaact tccctggtcg aacagttttt tctaatggct attcaagccc cagcatgcgc
     2581 ccagatgtaa gctctcctcc atccagctcc tcaacagcaa caacaggacc acctcccaaa
     2641 ctctgcctgg tgtgctctga tgaagcttca ggatgtcatt atggagtctt aacttgtgga
     2701 agctgtaaag ttttcttcaa aagagcagtg gaaggacagc acaattacct atgtgctgga
     2761 aggaatgatt gcatcatcga taaaattcga agaaaaaact gcccagcatg ccgctatcga
     2821 aaatgtcttc aggctggaat gaacctggaa gctcgaaaaa caaagaaaaa aataaaagga
     2881 attcagcagg ccactacagg agtctcacaa gaaacctctg aaaatcctgg taacaaaaca
     2941 atagttcctg caacgttacc acaactcacc cctaccctgg tgtcactgtt ggaggttatt
     3001 gaacctgaag tgttatatgc aggatatgat agctctgttc cagactcaac ttggaggatc
     3061 atgactacgc tcaacatgtt aggagggcgg caagtgattg cagcagtgaa atgggcaaag
     3121 gcaataccag gtttcaggaa cttacacctg gatgaccaaa tgaccctact gcagtactcc
     3181 tggatgtttc ttatggcatt tgctctgggg tggagatcat atagacaatc aagtgcaaac
     3241 ctgctgtgtt ttgctcctga tctgattatt aatgagcaga gaatgactct accctgcatg
     3301 tacgaccaat gtaaacacat gctgtatgtt tcctctgagt tacacaggct tcaggtatct
     3361 tatgaagagt atctctgtat gaaaacctta ctgcttctct cttcagttcc taaggacggt
     3421 ctgaagagcc aagagctatt tgatgaaatt agaatgacct acatcaaaga gctaggaaaa
     3481 gccattgtca agagggaagg aaactccagc cagaactggc agcggtttta tcaactgaca
     3541 aaactcttgg attctatgca tgaagtggtt gaaaatctcc ttaactattg cttccaaaca
     3601 tttttggata agaccatgag tattgaattc cccgagatgt tagctgaaat catcaccaat
     3661 cagataccaa aatattcaaa tggaaatatc aaaaaacttc tgtttcatca aaagtcaggt
     3721 taatagtgac tgcactcctc tggcaggtgg tcccacctgc ataagctgaa ctaagcctgt
     3781 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3841 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3901 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3961 agacaatagc aggcatgctg gggatgcggt gggctctatg gtatgcaaac ctttctaccg
     4021 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4081 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4141 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4201 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4261 cctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     4321 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     4381 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     4441 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     4501 ctcatctccg ggcctttcga cctaacagac catggccaag cctttgtctc aagaagaatc
     4561 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     4621 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     4681 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     4741 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     4801 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     4861 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     4921 ggagggctaa ggctccactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5041 ctcatcaatg tatcttatca tgtctggctc tgtatcagat gtacaaagtg gtactagtta
     5101 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     5161 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     5221 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     5281 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     5341 ccatcgacga gaaagggact gaagctgctg gggccatgtt tttagaggcc atacccatgt
     5401 ctatcccccc cgaggtcaag ttcaacaaac cctttgtctt cttaatgatt gaacaaaata
     5461 ccaagtctcc cctcttcatg ggaaaagtgg tgaatcccac ccaaaaataa ctgcctctcg
     5521 ctcctcaacc cctcccctcc atccctggcc ccctccctgg atgacattaa agaagggttg
     5581 agctggtccc tgcctgcatg tgactgtaaa tccctcccat gttttctctg agtctccctt
     5641 tgcctgctga ggctgtatgt gggctccagg taacagtgct gtcttcgggc cccctgaact
     5701 gtgttcatgg agcatctggc tgggtaggca catgctgggc ttgaatccag gggggactga
     5761 atcctcagct tacggacctg ggcccatctg tttctggagg gctccagtct tccttgtcct
     5821 gtctttgagt ccccaagaag gaatcacagg ggaggaacca gataccagcc atgaccccag
     5881 gctccaccaa gcatcttcat gtccccctgc tcatccccca ctccccccca cccagagttg
     5941 ctcatcctgc cagggctggc tgtgcccacc ccaaggctgc cctcctgggg accccagaac
     6001 tgcctgatcg tgccgtggcc cagttttgtg gcatctgcag caacacaaga gagaggacaa
     6061 tgtcctcctc ttgacccgct gtcacctaac cagactcggg ccctgcacct ctcaggcact
     6121 tctggaaaat gactgaggca gattcttcct gaagcccatt ctccatgggg caacaaggac
     6181 acctattctg tccttgtcct tccatcgctg ccccagaaag cctcacatat ctccgtttag
     6241 aatcaggtcc cttctcccca gatgaagagg agggtctctg ctttgttttc tctatctcct
     6301 cctcagactt gaccaggccc agcaggcccc agaagaccat taattaaggc cgggccgctc
     6361 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     6421 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     6481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6541 acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8641 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8701 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     8761 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     8821 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     8881 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     8941 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9001 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     9061 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     9121 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9181 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9241 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
     9301 cgtttaaacg gcgcgcctct gcacgacagg tctgccagct tacatttacc caaactgtcc
     9361 attactggaa cctatgatct gaagagcgtc ctgggtcaac tgggcatcac taaggtcttc
     9421 agcaatgggg ctgacctctc cggggtcaca gaggaggcac ccctgaagct ctccaaggtg
     9481 agatcaccct gacgaccttg ttgcaccctg gtatctgtag ggaagaatgt gtgggggctg
     9541 cagctctgtc ctgaggctga ggaaggggcc gagggaaaca aatgaagacc caggctgagc
     9601 tcctgaagat gcccgtgatt cactgacacg ggacgtggtc aaacagcaaa gccaggcagg
     9661 ggactgctgt gcagctggca ctttcggggc ctcccttgag gttgtgtcac tgaccctgaa
     9721 tttcaacttt gcccaagacc ttctagacat tgggccttga tttatccata ctgacacaga
     9781 aaggtttggg ctaagttgtt tcaaaggaat ttctgactcc ttcgatctgt gagatttggt
     9841 gtctgaatta atgaatgatt tcagctaaag atgacactta ttttggaaaa ctaaaggcga
     9901 ccaatgaaca actgcagttc catgaatggc tgcattatct tggggtctgg gcactgtgaa
     9961 ggtcactgcc agggtccgtg tcctcaagga gcttcaagcc gtgtactaga aaggagagag
    10021 ccctggaggc agacgtggag tgacgatgct cttccctgtt ctgagttgtg ggtgcacctg
    10081 agcaggggga gaggcgcttg tcaggaagat ggacagaggg gagccagccc catcagccaa
    10141 agccttgagg aggagcaagg cctatgtgac agggagggag aggatgtgca gggccagggc
    10201 cgtccagggg gagtgagcgc ttcctgggag gtgtccacgt gagccttgct cgaggcctgg
    10261 gatcagcctt acaacgtgtc tctgcttctc tcccctccag gccgtgcata aggctgtgtt
    10321 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
    10381 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
    10441 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
    10501 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
    10561 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
    10621 ttatagatat caactagaat gctagctgcc acctggtacc gagctcgact ttcacttttc
    10681 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
    10741 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
    10801 cttttctcta tcactgatag ggagtggtaa ac
//
