LOCUS       sequence-435139-        9717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van 
            de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, 
            Jacobs T, Eyckerman S
  TITLE     Leveraging a self-cleaving peptide for tailored control in proximity
            labeling proteomics.
  JOURNAL   Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9.
  PUBMED    38986614
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2024-07-15"; volume: "4(7):100818. doi"; pages: " 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9"
COMMENT     Sequence Label: pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH poly...
FEATURES             Location/Qualifiers
     source          1..9717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             107..505
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(548..567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     602..621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   849..911
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1934..1950
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1934..1950
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1984..2000)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1985..2001)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2030..2050)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2030..2048)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2069..2085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2069..2085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2082..2104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2093..2109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2117..2123,2124..2141,2142..2147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2162..2183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2300..2317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2471..3059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2551..2570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3230..4021,4022..4090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4091..4195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4221..4676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4358..4379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4570..4589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4803..4825
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4817..4834
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4818..4834
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4844..4863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4844..4862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   5898..5932
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(6254..6272,6273..6295,6296..6314,6315..6337,6338..6356,
                     6357..6379,6380..6398,6399..6421,6422..6440,6441..6463,
                     6464..6482,6483..6505,6506..6524)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     6551..6575
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6658..6667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6670..6711
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6718..7674
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             7690..7743
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    9069..9293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(9312..9717,1..94)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9347..9370)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
       61 gctgttctcc tcttcctcat ctccgggcct ttcgacctaa cagaccatgg ccaagccttt
      121 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
      181 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
      241 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
      301 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
      361 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
      421 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
      481 ctctggttat gtgtgggagg gctaaggctc cacttgttta ttgcagctta taatggttac
      541 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      601 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctgtat cagatgtaca
      661 aagtggtact agttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt
      721 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
      781 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
      841 ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta
      901 tctttctagg gttaaccatc gacgagaaag ggactgaagc tgctggggcc atgtttttag
      961 aggccatacc catgtctatc ccccccgagg tcaagttcaa caaacccttt gtcttcttaa
     1021 tgattgaaca aaataccaag tctcccctct tcatgggaaa agtggtgaat cccacccaaa
     1081 aataactgcc tctcgctcct caacccctcc cctccatccc tggccccctc cctggatgac
     1141 attaaagaag ggttgagctg gtccctgcct gcatgtgact gtaaatccct cccatgtttt
     1201 ctctgagtct ccctttgcct gctgaggctg tatgtgggct ccaggtaaca gtgctgtctt
     1261 cgggccccct gaactgtgtt catggagcat ctggctgggt aggcacatgc tgggcttgaa
     1321 tccagggggg actgaatcct cagcttacgg acctgggccc atctgtttct ggagggctcc
     1381 agtcttcctt gtcctgtctt tgagtcccca agaaggaatc acaggggagg aaccagatac
     1441 cagccatgac cccaggctcc accaagcatc ttcatgtccc cctgctcatc ccccactccc
     1501 ccccacccag agttgctcat cctgccaggg ctggctgtgc ccaccccaag gctgccctcc
     1561 tggggacccc agaactgcct gatcgtgccg tggcccagtt ttgtggcatc tgcagcaaca
     1621 caagagagag gacaatgtcc tcctcttgac ccgctgtcac ctaaccagac tcgggccctg
     1681 cacctctcag gcacttctgg aaaatgactg aggcagattc ttcctgaagc ccattctcca
     1741 tggggcaaca aggacaccta ttctgtcctt gtccttccat cgctgcccca gaaagcctca
     1801 catatctccg tttagaatca ggtcccttct ccccagatga agaggagggt ctctgctttg
     1861 ttttctctat ctcctcctca gacttgacca ggcccagcag gccccagaag accattaatt
     1921 aaggccgggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
     1981 tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag
     2041 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2101 cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct
     2161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4201 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4261 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4321 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4381 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4441 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4501 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4561 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4621 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     4681 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4741 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     4801 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     4861 gggcgaattg gagctcgttt aaacggcgcg cctctgcacg acaggtctgc cagcttacat
     4921 ttacccaaac tgtccattac tggaacctat gatctgaaga gcgtcctggg tcaactgggc
     4981 atcactaagg tcttcagcaa tggggctgac ctctccgggg tcacagagga ggcacccctg
     5041 aagctctcca aggtgagatc accctgacga ccttgttgca ccctggtatc tgtagggaag
     5101 aatgtgtggg ggctgcagct ctgtcctgag gctgaggaag gggccgaggg aaacaaatga
     5161 agacccaggc tgagctcctg aagatgcccg tgattcactg acacgggacg tggtcaaaca
     5221 gcaaagccag gcaggggact gctgtgcagc tggcactttc ggggcctccc ttgaggttgt
     5281 gtcactgacc ctgaatttca actttgccca agaccttcta gacattgggc cttgatttat
     5341 ccatactgac acagaaaggt ttgggctaag ttgtttcaaa ggaatttctg actccttcga
     5401 tctgtgagat ttggtgtctg aattaatgaa tgatttcagc taaagatgac acttattttg
     5461 gaaaactaaa ggcgaccaat gaacaactgc agttccatga atggctgcat tatcttgggg
     5521 tctgggcact gtgaaggtca ctgccagggt ccgtgtcctc aaggagcttc aagccgtgta
     5581 ctagaaagga gagagccctg gaggcagacg tggagtgacg atgctcttcc ctgttctgag
     5641 ttgtgggtgc acctgagcag ggggagaggc gcttgtcagg aagatggaca gaggggagcc
     5701 agccccatca gccaaagcct tgaggaggag caaggcctat gtgacaggga gggagaggat
     5761 gtgcagggcc agggccgtcc agggggagtg agcgcttcct gggaggtgtc cacgtgagcc
     5821 ttgctcgagg cctgggatca gccttacaac gtgtctctgc ttctctcccc tccaggccgt
     5881 gcataaggct gtgttaaccc tagaaagata gtctgcgtaa aattgacgca tgcattcttg
     5941 aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta ggacatctca
     6001 gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg
     6061 aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg acttttaaga
     6121 tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat aacttattat
     6181 atatatattt tcttgttata gatatcaact agaatgctag ctgccacctg gtaccgagct
     6241 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     6301 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     6361 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     6421 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     6481 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgacct
     6541 atataagcag agctcgttta gtgaaccgtc agatcgcctg cagacgccat ccacgctgtt
     6601 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttgaacagcc
     6661 accatgggcg gcaagcccat ccccaacccc ctgctgggcc tggacagcac cgctagcaaa
     6721 gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt ccatagtggc
     6781 gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca cattcagact
     6841 ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc tctgcccgag
     6901 cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca
     6961 gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag
     7021 agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa
     7081 tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga
     7141 ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag
     7201 ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag
     7261 ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt
     7321 ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg
     7381 atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc tacgctgatc
     7441 cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca
     7501 cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa
     7561 gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac
     7621 ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagggaggt
     7681 ggctccggag agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc
     7741 ccaggaggct cctctgataa tggaccccaa aatcagcgaa atgcaccccg cattacgttt
     7801 ggtggaccct cagattcaac tggcagtaac cagaatggag aacgcagtgg ggcgcgatca
     7861 aaacaacgtc ggccccaagg tttacccaat aatactgcgt cttggttcac cgctctcact
     7921 caacatggca aggaagacct taaattccct cgaggacaag gcgttccaat taacaccaat
     7981 agcagtccag atgaccaaat tggctactac cgaagagcta ccagacgaat tcgtggtggt
     8041 gacggtaaaa tgaaagatct cagtccaaga tggtatttct actacctagg aactgggcca
     8101 gaagctggac ttccctatgg tgctaacaaa gacggcatca tatgggttgc aactgaggga
     8161 gccttgaata caccaaaaga tcacattggc acccgcaatc ctgctaacaa tgctgcaatc
     8221 gtgctacaac ttcctcaagg aacaacattg ccaaaaggct tctacgcaga agggagcaga
     8281 ggcggcagtc aagcctcttc tcgttcctca tcacgtagtc gcaacagttc aagaaattca
     8341 actccaggca gcagtagggg aacttctcct gctagaatgg ctggcaatgg cggtgatgct
     8401 gctcttgctt tgctgctgct tgacagattg aaccagcttg agagcaaaat gtctggtaaa
     8461 ggccaacaac aacaaggcca aactgtcact aagaaatctg ctgctgaggc ttctaagaag
     8521 cctcggcaaa aacgtactgc cactaaagca tacaatgtaa cacaagcttt cggcagacgt
     8581 ggtccagaac aaacccaagg aaattttggg gaccaggaac taatcagaca aggaactgat
     8641 tacaaacatt ggccgcaaat tgcacaattt gcccccagcg cttcagcgtt cttcggaatg
     8701 tcgcgcattg gcatggaagt cacaccttcg ggaacgtggt tgacctacac aggtgccatc
     8761 aaattggatg acaaagatcc aaatttcaaa gatcaagtca ttttgctgaa taagcatatt
     8821 gacgcataca aaacattccc accaacagag cctaaaaagg acaaaaagaa gaaggctgat
     8881 gaaactcaag ccttaccgca gagacagaag aaacagcaaa ctgtgactct tcttcctgct
     8941 gcagatttgg atgatttctc caaacaattg caacaatcca tgagcagtgc tgactcaact
     9001 caggcctcag gttaatagtg actgcactcc tctggcaggt ggtcccacct gcataagctg
     9061 aactaagcct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9121 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9181 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9241 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggtatgcaa
     9301 acctttctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     9361 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     9421 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     9481 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     9541 ggaagtagca cgcctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     9601 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     9661 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcggg
//
