LOCUS       sequence-43514-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA8k-RFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(150..434)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(208..227)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 caccttcacc ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga
       61 aacgcatgaa ctctttgata acgtcttcgc tactcgccat atgtatatct ccttcttaaa
      121 agatcttttg aattcccaaa aaaacgggta tggagaaaca gtagagagtt gcgataaaaa
      181 gcgtcaggta gaatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac
      241 gccgtgcaaa taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt
      301 ttttgtcatg gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg
      361 tttggttagc gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg
      421 acaattggtt tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga
      481 tcccctgctg ccgggatact cgtttaatgc ccatctggtg gcgggtttaa cgccgattga
      541 ggccaacggt tatctcgatt tttttatcga ccgaccgctg ggaatgaaag gttatattct
      601 caatctcacc attcgcggtc agggggtggt gaaaaatcag ggacgagaat ttgtttgccg
      661 accgggtgat attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga
      721 ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg
      781 gcttaactgg gccg
//
