LOCUS       sequence-435142-        8750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van 
            de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, 
            Jacobs T, Eyckerman S
  TITLE     Leveraging a self-cleaving peptide for tailored control in proximity
            labeling proteomics.
  JOURNAL   Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9.
  PUBMED    38986614
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2024-07-15"; volume: "4(7):100818. doi"; pages: " 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9"
COMMENT     Sequence Label: pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH p...
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..206
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      289..298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..342
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             349..1305
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     polyA_signal    1695..1919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1938..2437
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1973..1996)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2370..2389
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2450..2848
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(2891..2910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2945..2964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3192..3254
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     4277..4293
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4277..4293
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4327..4343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4328..4344)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4373..4393)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4373..4391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4412..4428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4412..4428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4425..4447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4436..4452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4460..4466,4467..4484,4485..4490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4505..4526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4643..4660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4814..5402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4894..4913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5573..6364,6365..6433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6434..6538)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6564..7019)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6701..6722)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6913..6932
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7146..7168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7160..7177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7161..7177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7187..7206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7187..7205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8241..8275
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(8597..8615,8616..8638,8639..8657,8658..8680,8681..8699,
                     8700..8722,8723..8741,8742..8750,1..10,11..29,30..52,53..71,
                     72..94,95..113)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8639..8657,8658..8680,8681..8699,8700..8722,8723..8741,
                     8742..8750,1..10,11..29,30..52,53..71,72..94,95..113,
                     114..136,137..155)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
       61 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      121 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgacc tatataagca
      181 gagctcgttt agtgaaccgt cagatcgcct gcagacgcca tccacgctgt tttgacctcc
      241 atagaagaca ccgggaccga tccagcctcc gcggccccga attgaacagc caccatgggc
      301 ggcaagccca tccccaaccc cctgctgggc ctggacagca ccgctagcaa agacaatact
      361 gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg
      421 ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac
      481 tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg
      541 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
      601 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
      661 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
      721 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
      781 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
      841 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
      901 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
      961 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     1021 caggaagcag ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg
     1081 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag
     1141 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     1201 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     1261 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagggagg tggctccgga
     1321 gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaattttgg cccaggaggc
     1381 tcctctaaaa tgtcagatgt aaagtgcaca tcagtagtct tactctcagt tttgcaacaa
     1441 ctcagagtag aatcatcatc taaattgtgg gctcaatgtg tccagttaca caatgacatt
     1501 ctcttagcta aagatactac tgaagccttt gaaaaaatgg tttcactact ttctgttttg
     1561 ctttccatgc agggtgctgt agacataaac aagctttgtg aagaaatgct ggacaacagg
     1621 gcaaccttac aatcaggtta atagtgactg cactcctctg gcaggtggtc ccacctgcat
     1681 aagctgaact aagcctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1741 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1801 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1861 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     1921 atgcaaacct ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     1981 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     2041 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     2101 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     2161 acaaatggaa gtagcacgcc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     2221 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     2281 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     2341 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     2401 cgcgctgttc tcctcttcct catctccggg cctttcgacc taacagacca tggccaagcc
     2461 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     2521 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     2581 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     2641 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     2701 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     2761 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     2821 gccctctggt tatgtgtggg agggctaagg ctccacttgt ttattgcagc ttataatggt
     2881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctctg tatcagatgt
     3001 acaaagtggt actagttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt
     3061 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa
     3121 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga
     3181 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga
     3241 ttatctttct agggttaacc atcgacgaga aagggactga agctgctggg gccatgtttt
     3301 tagaggccat acccatgtct atcccccccg aggtcaagtt caacaaaccc tttgtcttct
     3361 taatgattga acaaaatacc aagtctcccc tcttcatggg aaaagtggtg aatcccaccc
     3421 aaaaataact gcctctcgct cctcaacccc tcccctccat ccctggcccc ctccctggat
     3481 gacattaaag aagggttgag ctggtccctg cctgcatgtg actgtaaatc cctcccatgt
     3541 tttctctgag tctccctttg cctgctgagg ctgtatgtgg gctccaggta acagtgctgt
     3601 cttcgggccc cctgaactgt gttcatggag catctggctg ggtaggcaca tgctgggctt
     3661 gaatccaggg gggactgaat cctcagctta cggacctggg cccatctgtt tctggagggc
     3721 tccagtcttc cttgtcctgt ctttgagtcc ccaagaagga atcacagggg aggaaccaga
     3781 taccagccat gaccccaggc tccaccaagc atcttcatgt ccccctgctc atcccccact
     3841 cccccccacc cagagttgct catcctgcca gggctggctg tgcccacccc aaggctgccc
     3901 tcctggggac cccagaactg cctgatcgtg ccgtggccca gttttgtggc atctgcagca
     3961 acacaagaga gaggacaatg tcctcctctt gacccgctgt cacctaacca gactcgggcc
     4021 ctgcacctct caggcacttc tggaaaatga ctgaggcaga ttcttcctga agcccattct
     4081 ccatggggca acaaggacac ctattctgtc cttgtccttc catcgctgcc ccagaaagcc
     4141 tcacatatct ccgtttagaa tcaggtccct tctccccaga tgaagaggag ggtctctgct
     4201 ttgttttctc tatctcctcc tcagacttga ccaggcccag caggccccag aagaccatta
     4261 attaaggccg ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     4321 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
     4381 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4441 atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa gcctggggtg
     4501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5221 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6541 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     6601 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6661 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6721 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6781 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6841 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6901 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6961 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7021 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7081 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7141 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     7201 atagggcgaa ttggagctcg tttaaacggc gcgcctctgc acgacaggtc tgccagctta
     7261 catttaccca aactgtccat tactggaacc tatgatctga agagcgtcct gggtcaactg
     7321 ggcatcacta aggtcttcag caatggggct gacctctccg gggtcacaga ggaggcaccc
     7381 ctgaagctct ccaaggtgag atcaccctga cgaccttgtt gcaccctggt atctgtaggg
     7441 aagaatgtgt gggggctgca gctctgtcct gaggctgagg aaggggccga gggaaacaaa
     7501 tgaagaccca ggctgagctc ctgaagatgc ccgtgattca ctgacacggg acgtggtcaa
     7561 acagcaaagc caggcagggg actgctgtgc agctggcact ttcggggcct cccttgaggt
     7621 tgtgtcactg accctgaatt tcaactttgc ccaagacctt ctagacattg ggccttgatt
     7681 tatccatact gacacagaaa ggtttgggct aagttgtttc aaaggaattt ctgactcctt
     7741 cgatctgtga gatttggtgt ctgaattaat gaatgatttc agctaaagat gacacttatt
     7801 ttggaaaact aaaggcgacc aatgaacaac tgcagttcca tgaatggctg cattatcttg
     7861 gggtctgggc actgtgaagg tcactgccag ggtccgtgtc ctcaaggagc ttcaagccgt
     7921 gtactagaaa ggagagagcc ctggaggcag acgtggagtg acgatgctct tccctgttct
     7981 gagttgtggg tgcacctgag cagggggaga ggcgcttgtc aggaagatgg acagagggga
     8041 gccagcccca tcagccaaag ccttgaggag gagcaaggcc tatgtgacag ggagggagag
     8101 gatgtgcagg gccagggccg tccaggggga gtgagcgctt cctgggaggt gtccacgtga
     8161 gccttgctcg aggcctggga tcagccttac aacgtgtctc tgcttctctc ccctccaggc
     8221 cgtgcataag gctgtgttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc
     8281 ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc
     8341 tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt
     8401 ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta
     8461 agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat
     8521 tatatatata ttttcttgtt atagatatca actagaatgc tagctgccac ctggtaccga
     8581 gctcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     8641 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     8701 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
//
