LOCUS       sequence-435143-       10551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct expressing msfGFP and nuclear mCherry 
            proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van 
            de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, 
            Jacobs T, Eyckerman S
  TITLE     Leveraging a self-cleaving peptide for tailored control in proximity
            labeling proteomics.
  JOURNAL   Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9.
  PUBMED    38986614
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2024-07-15"; volume: "4(7):100818. doi"; pages: " 
            10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9"
COMMENT     Sequence Label: pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
FEATURES             Location/Qualifiers
     source          1..10551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..29
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13..29
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     48..68
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        50..68
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        96..322
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             323..503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    550..675
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1168..1401
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1586..1630
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1896..2013
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2019..2039
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2046..3224
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2277..3215
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3172..3192
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3229..3238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3241..3261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3280..3282,3283..3993)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3322..3343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3930..3951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4009..4062
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4069..4776
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4214..4232)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4482..4501
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4783..4809
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    4832..5420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4885..4905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5303..5314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5430..5454
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5468..5967
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5503..5526)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             6347..6946
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6843..6863
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7074..7094)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             7165..7398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7470..7591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7507..7526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7561..7580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7631..7766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7786..7805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7787..7805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7815..7832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7815..7831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7824..7846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7973..8428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8060..8079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8270..8291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8454..8558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8559..8627,8628..9419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8777..8796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9590..10178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10079..10098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10332..10349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10466..10487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10502..10507,10508..10525,10526..10532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(10540..10551,1..5)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10545..10551,1..16)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
       61 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
      121 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      181 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      241 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      301 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      481 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      541 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      601 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      661 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      721 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      781 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      841 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      901 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      961 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1021 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1081 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1141 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1201 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1261 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1321 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1381 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1441 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1501 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1561 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1621 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1681 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1741 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1801 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     1861 tcgattagtg aacggatctc gacggtatcg ctagctttta aaagaaaagg ggggattggg
     1921 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     1981 ttacaaaaac aaattacaaa aattcaaaat tttactagca actttgtata gaaaagttgc
     2041 tacctggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     2101 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     2161 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     2221 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     2281 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     2341 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     2401 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     2461 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     2521 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     2581 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     2641 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     2701 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     2761 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     2821 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     2881 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     2941 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3001 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     3061 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     3121 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     3181 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaaacagc caccatgggc
     3241 cccaagaaga agagaaaggt gggacgcgtg agcggctctg tgagcaaggg cgaggagctg
     3301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3361 agcgtgcgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     3421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3481 gtgcagtgct tcagccgcta ccccgaccac atgaagcgcc acgacttctt caagtccgcc
     3541 atgcccgaag gctacgtcca ggagcgcacc atcagcttca aggacgacgg cacctacaag
     3601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     3721 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     3781 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     3901 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3961 gggatcactc acggcatgga cgagctgtac aagtcaggcg gctccggaga gggcagggga
     4021 agtcttctaa catgcgggga cgtggaggaa aatcccggcc ccctgctgat ggtgagcaag
     4081 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4141 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4201 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4261 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4321 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4381 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4441 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     4501 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4561 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4621 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4681 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4741 cgccactcca ccggcggcat ggacgagctg tacaagggat ccctgcctcc acttgaaaga
     4801 ctgacactgt aaactataat cgaattccga taatcaacct ctggattaca aaatttgtga
     4861 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4921 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4981 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5041 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5101 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5221 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5281 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5341 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5401 cctttgggcc gcctccccgc atcgggtatg caagtttgta caaaaaagca ggcttaacct
     5461 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     5521 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     5581 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     5641 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     5701 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     5761 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     5821 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggggcccgg
     5881 agggcctccc gggggcccgc attctgcacg cttcaaaagc gcacggctgc cgcgctgttc
     5941 tcctcttcct catctccggg cctttcgacc taacagccac catgaccgag tacaagccca
     6001 cggtgcgcct cgccacccgc gacgacgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     6061 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     6121 gggtgggctt ttggggcagc ggccaatagc agttttgctc cttcgctttc tgggctcaga
     6181 ggcggggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     6241 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     6301 tgttctcctc ttcctcatct ccgggccttt cgacctaaca gccaccatga ccgagtacaa
     6361 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6421 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6481 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6541 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6601 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6661 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6721 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6781 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaaa cctccgcgcc
     6841 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6901 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggct acctagaaaa
     6961 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7021 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtaccgt atgcaacttt
     7081 attatacata gttggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7141 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7201 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7261 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7321 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7381 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7441 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7621 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7681 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7741 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7801 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7861 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7921 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7981 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8041 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8101 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8161 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8221 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8281 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8341 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8401 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9481 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg
    10021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10201 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10261 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10321 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10381 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10441 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10501 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga t
//
