LOCUS       sequence-43515-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS8a-RFP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      147..218
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      234..261
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
       61 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc
      121 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      181 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      241 cttcgggtgg gcctttctgc gtttatacct agggtacggg ttttgctgcc cgcaaacggg
      301 ctgttctggt gttgctagtt tgttatcaga atcgcagatc cggcttcagc cggtttgccg
      361 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      421 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      481 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      541 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      601 cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat
      661 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatgtaac
      721 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      781 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      841 ttttgcgtga gccatgagaa cgaaccattg
//
