LOCUS       sequence-435157-        4782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 promoter 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_Promoter_4
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1093..1214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1104..1123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1158..1177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1462..1479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1633..2221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1713..1732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2421..3212,3213..3281))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3386..3434
                     /note="synthetic polyadenylation signal"
     misc_feature    3448..3539
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3488..3507
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4115..4146
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      4173..4182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4179..4782,1..1049)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
       61 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
      121 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
      181 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
      241 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
      301 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
      361 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
      421 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
      481 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
      541 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
      601 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
      661 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
      721 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
      781 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
      841 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
      901 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
      961 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     1021 ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc
     1081 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     1141 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1201 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1261 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     1321 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     1381 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     1441 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2041 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa
     2401 atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc
     2461 tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt
     2521 gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc
     2581 cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct
     2641 actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg
     2701 ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg
     2761 tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca
     2821 cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag
     2881 ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg
     2941 ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag
     3001 tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat
     3061 agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg
     3121 atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca
     3181 gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca
     3241 aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat
     3301 tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag
     3361 gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt
     3421 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     3481 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctg
     3541 gcctaactgg ccggtaccac cgaccagagt ctgaagacta ttcgcctttc ccaacacgga
     3601 cctccgccca tccaggcccg ggactatccc ttcgcggtgt agcggcagcc ggagacctgg
     3661 ctgaggaggc aaccgcgtag acacctccct gcttagaaaa caaacactga accagaccga
     3721 tcccagttgg agggttcgaa aatgttccag acagcctgtc gggaggggtt gttgttgctg
     3781 ttggactaaa tagctattcc tgattggtca tgtatagggt tttttaaggc gggtgggggg
     3841 aggagggggt agaggaaagg ctccaaacac ctgcaggttg ggggcggaaa gctgtttgcg
     3901 attccctgga ctggttggtc ggggacagga ggtaattccc agccattgac ccccatttct
     3961 ctctctccct ccctcttgcc ctgcctcttt ctctccaccc ctatctttcc tggaaactcg
     4021 ctttgggcgc ggcagatcgc ccaggaccac accgcagcga gctcgctagc ctcgaggata
     4081 tcaagatctg gcctcggcgg ccaagcttag acactagagg gtatataatg gaagctcgac
     4141 ttccagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
     4201 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     4261 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     4321 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     4381 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     4441 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     4501 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     4561 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     4621 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     4681 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     4741 gggacaaaac catcgccctg atcatgaaca gtagtggcag ta
//
