LOCUS       sequence-43516-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS8a-RFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(201..485)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(259..278)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ctttggtaac tttcngttta gcggtctggg taccttcgta cggacgacct tcaccttcac
       61 cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga
      121 actctttgat aacgtcttcg ctactcgcca tatgtatatc tccttcttaa aagatctttt
      181 gaattcccaa aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt
      241 agaatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa
      301 ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat
      361 ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag
      421 cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt
      481 ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct
      541 gccgggatac tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg
      601 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac
      661 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc g
//
