LOCUS       sequence-43518-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS8c-RFP
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(184..468)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(242..261)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttagcggtct gggtaccttc gtacggacga ccttcacctt caccttcgat ttcgaactcg
       61 tgaccgttaa cggaaccttc catacgaact ttgaaacgca tgaactcttt gataacgtct
      121 tcgctactcg ccatatgtat atctccttct taaaagatct tttgaattcc caaaaaaacg
      181 ggtatggaga aacagtagag agttgcgata aaaagcgtca ggtagaatcc gctaatctta
      241 tggataaaaa tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt
      301 ttctgccgtg attatagaca cttttgttac gcgtttttgt catggctttg gtcccgcttt
      361 gttacagaat gcttttaata agcggggtta ccggtttggt tagcgagaag agccagtaaa
      421 agacgcagtg acggcaatgt ctgatgcaat atggacaatt ggtttcttct ctgaatggcg
      481 ggagtatgaa aagtatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta
      541 atgcccatct ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta
      601 tcgaccgacc gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg
      661 tggtgaaaaa tcagggacga gaatttgttt gccgaccggg tgatattttg ctgttcccgc
      721 caggagagat tcatcactac ggtcgtcatc cggaggc
//
