LOCUS       sequence-43520-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS8k-RFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(211..495)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(269..288)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 agcggaccac ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct
       61 tcaccttcac cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg
      121 aaacgcatga actctttgat aacgtcttcg ctactcgcca tatgtatatc tccttcttaa
      181 aagatctttt gaattcccaa aaaaacgggt atggagaaac agtagagagt tgcgataaaa
      241 agcgtcaggt agaatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga
      301 cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg
      361 tttttgtcat ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg
      421 gtttggttag cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg
      481 gacaattggt ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg
      541 atcccctgct gccgggatac tcgtttaatg cccatctggt ggcgggttta acgccgattg
      601 aggccaacgg ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc
      661 tcaatctcac cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc
      721 gaccgggtga tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg
      781 aggctcgcga atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat
      841 ggcttaactg gccgtcatat
//
