LOCUS       sequence-43521-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE5a-RFP
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      149..220
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      236..263
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(501..520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
       61 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaaggat
      121 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
      181 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      241 accttcgggt gggcctttct gcgtttatac ctagggcgtt cggctgcggc gagcggtatc
      301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      781 taacaggatt agcagagcga ggtatg
//
