LOCUS       sequence-435218-       10197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV HA-MADD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Dou D, Aiken J, Holzbaur ELF
  TITLE     RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of 
            synaptic vesicle precursors.
  JOURNAL   J Cell Biol. 2024 Jun 3;223(6):e202307092. doi: 
            10.1083/jcb.202307092. Epub 2024 Mar 21.
  PUBMED    38512027
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-06-3"; volume: "223(6):e202307092. doi"; pages: 
            " 10.1083/jcb.202307092. Epub 2024 Mar 21"
COMMENT     Sequence Label: CMV HA-MADD
FEATURES             Location/Qualifiers
     source          1..10197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      950..959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             962..988
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      6484..6493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6497..6631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6534..6553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6588..6607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6700..7057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6700..6891
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6723..6743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6908..7043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7077..7094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7077..7093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7306..7761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7393..7412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7603..7624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7778..7797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7957..7975)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8043..8147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8148..8216,8217..9008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8366..8385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9179..9767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9668..9687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9921..9938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10055..10076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10091..10096,10097..10114,10115..10121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10129..10145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10134..10156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10153..10169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10153..10169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt cccgaaccga cagtcggtct cttcaccaag gccattcgcg ccaccatggg
      961 ctacccctat gatgtgcctg actacgcaga tctcaatggt ggtggtggtg ggtcgaagct
     1021 tatggtgcaa aagaagaagt tctgtcctcg gttacttgac tatctagtga tcgtaggggc
     1081 caggcacccg agcagtgata gcgtggccca gactcctgaa ttgctacggc gatacccctt
     1141 ggaggatcac actgagtttc ccctgccccc agatgtagtg ttcttctgcc agcccgaggg
     1201 ctgcctgagc gtgcggcagc ggcgcatgag ccttcgggat gatacctctt ttgtcttcac
     1261 cctcactgac aaggacactg gagtcacgcg atatggcatc tgtgttaact tctaccgctc
     1321 cttccaaaag cgaatctcta aggagaaggg ggaaggtggg gcagggtccc gtgggaagga
     1381 aggaacccat gccacctgtg cctcagaaga gggtggcact gagagctcag agagtggctc
     1441 atccctgcag cctctcagtg ctgactctac ccctgatgtg aaccagtctc ctcggggcaa
     1501 acgccgggcc aaggcgggga gccgctcccg caacagtact ctcacgtccc tgtgcgtgct
     1561 cagccactac cctttcttct ccaccttccg agagtgtttg tatactctca agcgcctggt
     1621 ggactgctgt agtgagcgcc ttctgggcaa gaaactgggc atccctcgag gcgtacaaag
     1681 ggacaccatg tggcggatct ttactggatc gctgctggta gaggagaagt caagtgccct
     1741 tctgcatgac cttcgagaga ttgaggcctg gatctatcga ttgctgcgct ccccagtacc
     1801 cgtctctggg cagaagcgag tagacatcga ggtcctaccc caagagctcc agccagctct
     1861 gacctttgct cttccagacc catctcgatt caccctagtg gatttcccac tgcaccttcc
     1921 cttggaactt ctaggtgtgg acgcctgtct ccaggtgcta acctgcattc tgttagagca
     1981 caaggtggtg ctacagtccc gagactacaa tgcactctcc atgtctgtga tggcattcgt
     2041 ggcaatgatc tacccactgg aatatatgtt tcctgtcatc ccgctgctac ccacctgcat
     2101 ggcatcagca gagcagctgc tgttggctcc aaccccgtac atcattgggg ttcctgccag
     2161 cttcttcctc tacaaactgg acttcaaaat gcctgatgat gtatggctag tggatctgga
     2221 cagcaatagg gtgattgccc ccaccaatgc agaagtgctg cctatcctgc cagaaccaga
     2281 atcactagag ctgaaaaagc atttaaagca ggccttggcc agcatgagtc tcaacaccca
     2341 gcccatcctc aatctggaga aatttcatga gggccaggag atcccccttc tcttgggaag
     2401 gccttctaat gacctgcagt ccacaccgtc cactgaattc aacccactca tctatggcaa
     2461 tgatgtggat tctgtggatg ttgcaaccag ggttgccatg gtacggttct tcaattccgc
     2521 caacgtgctg cagggatttc agatgcacac gcgtaccctg cgcctctttc ctcggcctgt
     2581 ggtagctttt caagctggct cctttctagc ctcacgtccc cggcagactc cttttgccga
     2641 gaaattggcc aggactcagg ctgtggagta ctttggggaa tggatcctta accccaccaa
     2701 ctatgccttt cagcgaattc acaacaatat gtttgatcca gccctgattg gtgacaagcc
     2761 aaagtggtat gctcatcagc tgcagcctat ccactatcgc gtctatgaca gcaattccca
     2821 gctggctgag gccctgagtg taccaccaga gcgggactct gactccgaac ctactgatga
     2881 tagtggcagt gatagtatgg attatgacga ttcaagctct tcttactcct cccttggtga
     2941 ctttgtcagt gaaatgatga aatgtgacat taatggtgat actcccaatg tggaccctct
     3001 gacacatgca gcactggggg atgccagcga ggtggagatt gacgagctgc agaatcagaa
     3061 ggaagcagaa gagcctggcc cagacagtga gaactctcag gaaaacaccc cactgcgctc
     3121 cagctctagc accacagcca gcagcagccc cagcactgtc atccacggag ccaactctga
     3181 acctgctgac tctacggaga tggatgataa ggcagcagta ggcgtctcca agcccctccc
     3241 ttccgtgcct cccagcattg gcaaatcgaa cgtggacaga cgtcaggcag aaattggaga
     3301 gggggctcaa aagctgctgc ggcccaacag cttgagactg gcaagtgact cagatgcaga
     3361 gtcagactct cgggcaagct ctcccaactc caccgtctcc aacaccagca ccgagggctt
     3421 cgggggcatc atgtcttttg ccagcagcct ctatcggaac cacagtacca gcttcagtct
     3481 ttcaaacctc acactgccca ccaaaggtgc ccgagagaag gccacgccct tccccagtct
     3541 gaaaggaaac aggagggcgt tagtggatca gaagtcatct gtcattaaac acagcccaac
     3601 agtgaaaaga gaacctccat caccccaggg tcgatccagc aattctagtg agaaccagca
     3661 gttcctgaag gaggtggtgc acagcgtgct ggacggccag ggagttggct ggctcaacat
     3721 gaaaaaggtg cgcgggctgc tggagagcga gcagctgcga gtctttgtcc tgagcaagct
     3781 gaaccgcatg gtgcagtcag aggacgatgc ccggcaggac atcatcccgg atgtggagat
     3841 cagtcggaag gtgtacaagg gaatgttaga cctcctcaag tgtacagtcc tcagcttgga
     3901 gcagtcctat gcccacgcgg gtctgggtgg catggccagc atctttgggt ttttggagat
     3961 tgcccagacc cactactata gtaaagaacc agacaagcgg aagagaagtc caacagaaag
     4021 tgtaaatacc ccagttggca aggatcctgg cctagctggg cggggggacc caaaggctat
     4081 ggcacaactg agagttccac aactgggacc tcgggcacca agtgccacag gaaagggtcc
     4141 taaggaactg gacaccagaa gtttaaagga agaaaatttt atagcatcta ttgggcctga
     4201 agtaatcaaa cctgtctttg accttggtga gacagaggag aaaaagtccc agatcagcgc
     4261 agacagtggt gtgagcctga cgtctagttc ccagaggact gatcaagact ctgtcatcgg
     4321 cgtgagtcca gctgttatga tccgcagctc aagtcaggat tctgaagtta gcaccgtgag
     4381 taatagctct ggagagaccc ttggagctga cagtgacttg agcagcaatg caggtgatgg
     4441 accaggtggc gagggcagtg ttcacctggc aagctctcgg ggcactttgt ctgatagtga
     4501 aattgagacc aactctgcca caagcactat ctttggtaaa gcccacagct tgaagccaag
     4561 cataaaggag aagctggcag gcagccccat tcgtacttct gaagatgtga gccagcgagt
     4621 ctatctctat gagggactcc taggcaaaga gcgttctact ttatgggacc aaatgcaatt
     4681 ctgggaagat gccttcttag atgctgtgat gttggagaga gaagggatgg gtatggacca
     4741 gggtccccag gaaatgatcg acaggtacct gtcccttgga gaacatgacc ggaagcgcct
     4801 ggaagatgat gaagatcgct tgctggccac acttctgcac aacctcatct cctacatgct
     4861 gctgatgaag gtaaataaga atgacatccg caagaaggtg aggcgcctaa tgggaaagtc
     4921 gcacattggg cttgtgtaca gccagcaaat caatgaggtg cttgatcagc tggcgaacct
     4981 gaatggacgc gatctctcta tctggtccag tggcagccgg cacatgaaga agcagacatt
     5041 tgtggtacat gcagggacag atacaaacgg agatatcttt ttcatggagg tgtgcgatga
     5101 ctgtgtggtg ttgcgtagta acatcggaac agtgtatgag cgctggtggt acgagaagct
     5161 catcaacatg acctactgtc ccaagacgaa ggtgttgtgc ttgtggcgta gaaatggctc
     5221 tgagacccag ctcaacaagt tctatactaa aaagtgtcgg gagctgtact actgtgtgaa
     5281 ggacagcatg gagcgcgctg ccgcccgaca gcaaagcatc aaacccggac ctgaattggg
     5341 tggcgagttc cctgtgcagg acctgaagac tggtgagggt ggcctgctgc aggtgaccct
     5401 ggaagggatc aacctcaaat tcatgcacaa tcaggttttc atagagctga atcacattaa
     5461 aaagtgcaat acagttcgag gcgtctttgt cctggaggaa tttgttcctg aaattaaaga
     5521 agtggtgagc cacaagtaca agacaccaat ggcccacgaa atctgctact ccgtattatg
     5581 tctcttctcg tacgtggctg cagttcatag cagtgaggaa gatctcagaa ccccgccccg
     5641 gcctgtctct agctgatgga gaggggctac gcggctgccc cagcccaggg cacgcccctg
     5701 gccccttgct gttcccaagt gcacgatgct gctgtgactg aggagtggat gatgctcgtg
     5761 tgtcctctgc aagccccctg ctgtggcttg gttggttacc ggttatgtgt ccctctgagt
     5821 gtgtcttgag cgtgtccacc ttctccctct ccactcccag aagaccaaac tgccttcccc
     5881 tcagggctca agaatgtgta cagtctgtgg ggccggtgtg aacccactat tttgtgtcct
     5941 tgagacattt gtgttgtggt tccttgtcct tgtccctggc gttataactg tccactgcaa
     6001 gagtctggct ctcccttctc tgtgacccgg catgactggg cgcctggagc agtttcactc
     6061 tgtgaggagt gagggaaccc tggggctcac cctctcagag gaagggcaca gagaggaagg
     6121 gaagaattgg ggggcagccg gagtgagtgg cagcctccct gcttccttct gcattcccaa
     6181 gccggcagct actgcccagg gcccgcagtg ttggctgctg cctgccacag cctctgtgac
     6241 tgcagtggag cggcgaattc cctgtggcct gccacgcctt cggcatcaga ggatggagtg
     6301 gtcgaggcta gtggagtccc agggaccgct ggctgctctg cctgagcatc agggaggggg
     6361 caggaaagac caagctgggt ttgcacatct gtctgcaggc tgtctctcca ggcacggggt
     6421 gtcaggaggg agagacagcc tgggtatggg caagaaatga ctgtaaatgg gatccccagc
     6481 ttggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6601 actcatcaat gtatcttatc atgtctggat cgggaattaa ttcggcgcag caccatggcc
     6661 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     6721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6961 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7021 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgttaacag cttggcactg
     7081 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7141 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7201 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     7261 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     7321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7501 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     7561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7621 ctggaacaac actcaaccct atctcgggct attcttttaa tttataaggg attttgccga
     7681 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7741 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     7801 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7861 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7921 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7981 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8281 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8701 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9961 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10021 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10081 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10141 aacaatttca cacaggaaac agctatgaca tgattacgaa ttaattcgag ctcgccc
//
