LOCUS       sequence-43522-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE5a-RFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    208..224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(232..238,239..256,257..262))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(348..367)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        573..650
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(670..689)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga
       61 cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga
      121 actttgaaac gcatgaactc tttgataacg tcttcgctac tcgccatatg tatatctcct
      181 tcttaaaaga tcttttgaat tctgaaattg ttatccgctc acaattccac acattatacg
      241 agccggaagc ataaagtgta aagcctgggg tgcctaaacg atctcgatcc tctacgccgg
      301 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      361 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      421 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      481 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
      541 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
      601 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
      661 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
      721 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
      781 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
      841 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaa
//
