LOCUS       sequence-435256-        9487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger
            S, Erdjument-Bromage H, Tempst P, Spiegelman BM
  TITLE     Suppression of mitochondrial respiration through recruitment of p160
            myb binding protein to PGC-1alpha: modulation by p38 MAPK.
  JOURNAL   Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26.
  PUBMED    14744933
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2004-02-1"; volume: "18(3)"; pages: "278-89. Epub 2004 Jan 
            26"
COMMENT     Sequence Label: pcDNA-p160MBP
FEATURES             Location/Qualifiers
     source          1..9487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3005..3016
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             4991..5020
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5036..5053
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5076..5093)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5082..5306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5352..5780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5439..5458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5649..5670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5789..5809)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5794..6123
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5974..6109
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6036..6055
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6190..6984
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6244..6263)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6854..6873
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7160..7281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7197..7216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7251..7270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7330..7346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7330..7346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7343..7365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7354..7370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7378..7384,7385..7402,7403..7408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7423..7444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7561..7578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7732..8320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7812..7831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8491..9282,9283..9351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9352..9456)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttacc accatggcgg agatgaagag ccctacgaaa gctgagcctg cgactcccgc
      961 agaagcggcg caaagcgacc gccacagcct gctggagcac agccgcgagt tcttggactt
     1021 cttctgggac attgcgaaac cggatcagga aacgcggctc cgggccacgg agaagttgtt
     1081 ggagtacttg cgcacaaggc ccaatgattc ggagatgaaa tatgccctga agcgcctaat
     1141 cactgggctt ggggtgggcc gagaagccgc taggccctgc tacagcctgg cgctggcaca
     1201 gctgttgcag tcttttgaag acatcccatt gtgtgacatc ctggatcaga tacaagaaaa
     1261 atacagccta caagccatga acaaggcaat gatgagacct agtctctttg caaacctttt
     1321 tggagtgcta gccctctttc agtcaggccg cctagtgaag gacaaagagg ccctgatgaa
     1381 gtccgtgcaa ttgctgaaga tcctgtccca acaccccaac cacttacagg gacagccaat
     1441 aaaggctctg gtggacatcc tctctgaggt cccagagtcc atgttccagg agatcctgcc
     1501 aaaggtcctc aagggtaaca tgaaagtgat cctccgctct cccaagtact tggagctctt
     1561 cctcctggct aagcagaggg tgccgacaaa gctcgagtca ctcatgggct cggttgacct
     1621 attctcagaa gacaatattc ccagtctggt gaacatcctg aaggtggccg ccaactctgt
     1681 caagaaggag cacaagctgc ctaatgtggc tctggacctg ctccgcctgg ctctcaagga
     1741 gagcagattc gaactgttct ggaagaaggt tttggaggag gggctgctga agaatccgtc
     1801 ctggacatcc agctacatgt gcttccgcct actgggtgcg tctctgccgc ttctgtcaga
     1861 ggagcagttg cagttggtga tgcgaggaga cttgatccgc cattttgggg agaacatggt
     1921 tatttctaag ccccaaaacc tatttaagat catcccagag ataagtacat acgtgggtac
     1981 cttcctagag gggtgccagg atgaccctaa gcggcagttg actatgatgg tggcctttac
     2041 aaccatcacc aatcaaggtc tccctgtcat gcctaccttc tggcgtgtca cgcggttttt
     2101 gaatgctgaa gccctgcaga gctatgtggc ctggttgcgg gacatgttcc tgcagcctga
     2161 cctgaactcc ttggttgact tcagcactgc caaccagaag agagctcagg acgcctcgtt
     2221 gaatgtgcct gagcgagctg tattccggct ccggaagtgg atcatccacc gcctggtcag
     2281 ccttgtggat catttgcatc tggagaagga tgaagctgtg gttgagcaaa tagccaggtt
     2341 ttgcttgttc catgccttct ttaagacgaa gaaggctacg ccccagatcc cagagacgaa
     2401 gcagcacttc tccttccctt tggacgaccg caaccgtggg gtctttgtca gtgccttctt
     2461 cagcctactg cagacgctca gtgtgaagtt caggcagaca ccagacctgg ctgaaaatgg
     2521 gaagccttgg acttaccgcc tggttcagtt ggcagacatg ctgttgaacc ataaccgcaa
     2581 tgtaaccagt gtgacatcct tgacaacaca gcagcgtcag gcctgggacc agatgatgag
     2641 tactctgaag gaattagagg cccgctcctc tgagaccagg gccattgcct tccagcacct
     2701 gctgcttctg gtgggcctcc acatcttcaa gtcccctgca gaaagctgtg atgtcctagg
     2761 agacattcag acttgcatca agaaaagcat ggagcagaat ccccgccgat cacgctctag
     2821 agccaaagcc tcccaggagc cagtatgggt ggaggtgatg gtggagatct tgctgtcctt
     2881 gctggctcaa cccagcaact tgatgcgcca ggtggtccgg agtgtatttg gtcatatctg
     2941 tccccacctt actccacgtt gtctgcagct aatcctggct gtgctcagcc ctgtgacaaa
     3001 cgaggatgag gatgacaacg tggtggtcac tgatgacgct gatgagaagc agctgcagca
     3061 tggagaggac gaagactcag ataatgagga caataagaac tcagagagtg acatggacag
     3121 tgaggatggg gaagaaagtg aagaggagga ccgtgacaaa gatgtggacc caggcttccg
     3181 tcaacagttg atggaagtgt taaaagctgg gaatgcattg ggtggagtgg acaacgagga
     3241 ggaggaggag cttggggatg aggccatgat ggccctggac cagaacctgg ccagcctatt
     3301 taaagagcag aagatgcgca tccaggcccg gaatgaggag aaaaacaagc tacagaagga
     3361 gaagaagctc cgacgggact tccaaatcag ggcactagac ctgatcgagg tgctggtgac
     3421 caagcagcct gagcaccccc tgatcctgga actacttgag ccactgctga acgtgatcca
     3481 gcacagcatg cgcagcaaag gctccaccaa gcaggagcag gacctcctgc acaagaccgc
     3541 ccgcatcttc atgcaccacc tgtgtcgtgc ccgccgctac tgccacgagg tggggccgtg
     3601 tgcagaggct ctgcatgccc aggtggagag gcttgtgcag caggctggca gccaggctga
     3661 tgcctctgtc gccctatact atttcaatgc ctctctgtac ctgctgcgag tcctcagggg
     3721 caacaccaat aagaggcacc aagatggtca taagttacac ggagctgaca cagaggactc
     3781 agaggaccag gctgctaact gcttagactt ggactttgtg acccgggtgt attcagcatc
     3841 actggaatct cttctgacca agcgtaacag ctcacttacg gtccccatgt tcctcagcct
     3901 cttctccaga tacccagtga tctgtaagaa cctgcttccc gtcctggctc agcatgtggc
     3961 tggcccatct cggccccgcc atcaggccca ggcatgcctg atgctccaga agactctgtc
     4021 tgcacgagag ctgagagtct gttttgagga tcctgagtgg gagcagctga ttacccaact
     4081 cttgggaaag gccacccaga ccctgcagac tcttggggag gcacagagca agggggagca
     4141 ccagaaggag ctatccatct tggagctgct gaacactctg ttgaggacag tcaatcacga
     4201 gaagctgtct gtggacctca ctgctccctt gggcgtgctt cagagcaagc aacagaagct
     4261 gcagcaaagc ctgcagcagg ggaatcactc atctggctcc aatcgcctct atgatctcta
     4321 ctggcaggcc atgaggatgc taggagtcca acgtccaaag tcagaaaaga agaatgccaa
     4381 ggatattcct agtgacaccc agagccccgt cagcacaaag cggaagaaaa agggattctt
     4441 gccagagacc aagaagcgaa agaaacttaa atctgagggc accacaccag aaaagaatgc
     4501 tgcgtcacag caggatgcag tgacagaggg tgccatgcct gctgccactg gtaaagacca
     4561 gccccccagc acaggcaaga agaaaaggaa gagggtaaag gccagcaccc catcccaggt
     4621 gaatgggata actggggcca agagtccagc tcccagtaac cccaccctaa gccccagcac
     4681 ccctgccaag accccaaaac tgcagaagaa aaaagagaag ctgtcacagg tgaatggagc
     4741 cactcctgtg tcccccatag agcctgaaag caaaaagcat catcaggagg cactcagcac
     4801 aaaggaggtc ataagaaagt ccccccaccc ccagtctgcc ctgccaaaga aaagagcaag
     4861 gctgtctctg gtgagcagga gccccagcct gttacagagt ggggtcaaga aaaggagagt
     4921 ggccagcagg agagtgcaga cacctagata tccagcacag tggcggccgc tcgagtctag
     4981 agggcccttc gaacaaaaac tcatctcaga agaggatctg aatatgcata ccggtcatca
     5041 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5101 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5161 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5221 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5281 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5341 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5401 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5461 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5521 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5581 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5641 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5701 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5761 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5821 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5881 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5941 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6001 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6061 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6121 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     6181 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6241 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6301 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6361 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6421 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6481 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6541 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6601 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6661 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6721 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6781 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6841 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6901 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6961 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     7021 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7081 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7141 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7261 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7321 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7441 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7501 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7561 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7621 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7681 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7741 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7801 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7861 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7921 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7981 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8041 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8101 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8161 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8221 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8281 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8341 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8401 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8461 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8521 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8581 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8641 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8701 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8761 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8821 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8881 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8941 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9001 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9061 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9121 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9181 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9241 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9301 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9361 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9421 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9481 tgacgtc
//
