LOCUS       sequence-43527-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA5a-RFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      150..221
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      237..264
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(292..309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(506..525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
       61 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaagga
      121 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
      181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      241 caccttcggg tgggcctttc tgcgtttata cctagggata tattccgctt cctcgctcac
      301 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
      361 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
      421 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
      481 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc
      541 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
      601 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
      661 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
      721 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
      781 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
      841 ac
//
