LOCUS       sequence-435278-        6789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB714
FEATURES             Location/Qualifiers
     source          1..6789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1157..1344
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1212..1236)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1820..2227
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2228..3322
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4353..5168)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4353..5168)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5076..5095
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5160..5179
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5393..5981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5882..5901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg tttcgaagaa
      721 aaatacggct gaaatcagtg caaaagatat atgggagaat atatggagtg gtgtaagttc
      781 gctgcttgat ttcttcgcag tattggaaaa cctaggcgtg gtcaacgata agttatatgt
      841 aagtgggcta ttacggaaag tttggctgtg ttactcgtgc atttcagtga taaaatgtgt
      901 atggaaatta ataaaattat gcaaggtgaa atttaaaatc gatcaacgat tggatgggga
      961 gggaaatggt ctagtaaagg ataagttgat aaacttcaag aaaaaatata acgaacatat
     1021 cagacatatt actgctgctt tactccaaga tttgagctat ttaatggttt taatctatcc
     1081 agggacgagg ttatttaaaa gattatctaa cataataaca ctttgtagaa taattgtata
     1141 aggatcctaa ctcgaggcga atttcttatg atttatgatt tttattatta aataagttat
     1201 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     1261 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     1321 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt
     1381 cgctggaaat ctgctcgtca gtggtgctca cactgacgaa tcatgtacag atcataccga
     1441 tgactgcctg gcgactcaca actaagcaag acagccggaa ccagcgccgg cgaacaccac
     1501 tgcatatatg gcatatcaca acagtccacg tctcaagcag ttacagagat gttacgaacc
     1561 actagtgcac tgcagtacat cgaggagaac ttctagtata tctacatacc taatattatt
     1621 gccttattaa aaatggaatc ccaacaatta catcaaaatc cacattctct tcaaaatcaa
     1681 ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt atatttatag gataattata
     1741 ctctatttct caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag
     1801 aaatatcttg accgcagtta actgtgggaa tactcaggta tcgtaagatg caagagttcg
     1861 aatctcttag caaccattat ttttttcctc aacataacga gaacacacag gggcgctatc
     1921 gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc gcctgacgca
     1981 tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagagcgc cggaaccggc
     2041 ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat acttgaagtt
     2101 gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat cgtcttactt
     2161 tctaactttt cttacctttt acatttcagc aatatatata tatatatttc aaggatatac
     2221 cattctaatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga
     2281 aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa
     2341 gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgttccact
     2401 tccagatgag gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg
     2461 tggtcctaaa tggggaaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa
     2521 agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga
     2581 cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt
     2641 agtgggaggt atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga
     2701 tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc
     2761 cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc
     2821 ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa
     2881 ggttcaacat caattgattg attctgccgc catgatccta gttaagaacc caacccacct
     2941 aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt
     3001 tatcccaggt tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa
     3061 caccgcattt ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa
     3121 ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt
     3181 gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag
     3241 aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga
     3301 agttaagaaa atccttgctt aaaaagattc tcttttttta tgatatttgt acataaactt
     3361 tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg
     3421 tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatgt
     3481 aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg
     3541 cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag gtgtaacaga
     3601 aaatcatgaa actatgattc ctaatttata tattggagga ttttctctaa aaaaaaaaaa
     3661 atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa gtcaatacct
     3721 tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct gtcagaaacg
     3781 gccttaacga cgtagtcgag agtactcgta tcgcatgtcg gtgcgacacg aaattacaaa
     3841 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
     3901 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
     3961 tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca
     4021 aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat
     4081 tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
     4141 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
     4201 ttttactctg tcagaaacgg ccttaacgac gtagtcgacc tcctcttcag tactaaatct
     4261 accaatacca aatctgatgg aagaatgggc taatgcatca tccttaccca gcgccgagcg
     4321 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     4381 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     4441 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     4501 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     4561 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     4621 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     4681 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
     4741 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     4801 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     4861 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     4921 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     4981 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     5041 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     5101 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
     5161 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     5221 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     5281 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
     5341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5521 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6061 ctgattctgt ggataaccgt gcggccgcca atacccagaa gaacagtaaa ataaagcaag
     6121 gtacgtgaaa ttaatatttt taaatggttc taaccgatgc cgaagaactg cgcagtccgg
     6181 ttataacgtc tgacatgtcc ttttttgatt tggaatccaa ccactcaagt gactctgttc
     6241 atttactttg cgaaaaatat acccacaaat tgcccatcga aagtgaatcg caaaccacct
     6301 tcagactggc accgacaaag caaagattat acagacagag tactttatac gtaccgttaa
     6361 gtctcaagca aagggttttc ttatttactg aacgggtaaa gagtatctgg gccggcttgc
     6421 caagatgcaa accgaataag tatttcaaag ttgcatttgc cttagccgtc ctgacaccat
     6481 tggctatttg gatattttat attgactttc gtgtacattg atcacatcga ctgttctatt
     6541 ggcaaatgaa ccacgggcat tgactatttt tcaggttact actatatatt atcatcacgg
     6601 gcaaggattg taccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag
     6661 ccggtacaag caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg
     6721 acggcgatca cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc
     6781 aatacaacg
//
