LOCUS       sequence-43528-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA5a-RFP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(231..237,238..255,256..261))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(347..366)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        572..649
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(669..688)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 acggcagcgg accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac
       61 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa
      121 ctttgaaacg catgaactct ttgataacgt cttcgctact cgccatatgt atatctcctt
      181 cttaaaagat cttttgaatt ctgaaattgt tatccgctca caattccaca cattatacga
      241 gccggaagca taaagtgtaa agcctggggt gcctaaacga tctcgatcct ctacgccgga
      301 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      361 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      421 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      481 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
      541 gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg
      601 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
      661 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
      721 gaaccangcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
      781 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
      841 tggcgttgcc acctccagt
//
