LOCUS       sequence-43530-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA5c-RFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    193..209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(217..223,224..241,242..247))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(333..352)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        558..635
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(655..674)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
       61 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
      121 aactctttga taacgtcttc gctactcgcc atatgtatat ctccttctta aaagatcttt
      181 tgaattctga aattgttatc cgctcacaat tccacacatt atacgagccg gaagcataaa
      241 gtgtaaagcc tggggtgcct aaacgatctc gatcctctac gccggacgca tcgtggccgg
      301 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      361 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      421 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      481 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
      541 agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat
      601 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
      661 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac c
//
