LOCUS       sequence-43533-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS5a-RFP
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      141..212
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      228..255
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
       61 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg atccaaactc
      121 gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      241 gtgggccttt ctgcgtttat acctagggta cgggttttgc tgcccgcaaa cgggctgttc
      301 tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt gccggctgaa
      361 agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc actggctccc
      421 gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg tctatgtgtg
      481 actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt
      541 tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct
      601 gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct
      661 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa
      721 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
      781 gaacctcaga tccttccgta tttagccagt atgttctc
//
