LOCUS       sequence-435356-       14165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under 
            regulation of CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Dombey R, Buendia-Avila D, Barragan-Borrero V, Diezma-Navas L, 
            Ponce-Mane A, Vargas-Guerrero JM, Elias R, Mari-Ordonez A
  TITLE     Atypical epigenetic and small RNA control of degenerated transposons
            and their fragments in clonally reproducing Spirodela polyrhiza
  JOURNAL   bioRxiv 2024.04.03.587901; doi: 
            https://doi.org/10.1101/2024.04.03.587901
REFERENCE   2  (bases 1 to 14165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.03.587901; doi: https://doi.org/10.1101/2024.04.03.587901"
COMMENT     Sequence Label: p35S-FHA-AtAGO4_gDNA
FEATURES             Location/Qualifiers
     source          1..14165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    155..179
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    247..267
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        270..288
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     270..287
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        948..1292
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1236..1256
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1393..1417
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1423..1446
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1462..1488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6210..6234)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6247..6256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6621..6800
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6842..7393
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      7413..7665
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7758..7782
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8303..9094
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9340..9928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9829..9848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10082..10099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10114..10254
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10180..10199)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10340..10362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10598..10792
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10858..11931)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12360..12989)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tcaaagtact ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct
       61 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
      121 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
      181 acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct
      241 tggctccaac tttgtataga aaagttgtca tttaggtgac actatagaat actcaagcta
      301 tgcatccaac gcgttgggag ctctcccata tggtcgacta gagccaagct gatctccttt
      361 gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac
      421 ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc
      481 agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct catcaagacg
      541 atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca
      601 gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc
      661 agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata
      721 gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt
      781 cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
      841 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc
      901 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
      961 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     1021 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
     1081 gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     1141 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     1201 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     1261 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa
     1321 caaaacaaac aacaaacaac attacaattt actattctag tcgacctgca ggcggccgca
     1381 ctagtgatat cagcaagttt gtacaaaaaa gcaggcttca tggattacaa ggatgacgat
     1441 gacaagggag gtggcggatc ctacccatac gatgttccag attacgctgg aggcggtgat
     1501 tcaacaaatg gtaacggagc tgatcttgaa tcagcaaatg gggcaaacgg gagtggggtt
     1561 actgaggcat taccacctcc tccaccagtt atacctccaa atgtggaacc agttcgtgtt
     1621 aaaactgaac ttgctgagaa gaaggggcca gttcgagttc ctatggctcg aaaaggattt
     1681 ggaacaaggg gccaaaagat ccccttgtta acaaatcatt tcaaagtcga tgtggctaat
     1741 cttcagggtc atttcttcca ctacagtgta agtttttgaa actattttgt gttatttttg
     1801 ttggatgatt gatgtcacta gaatggatgc agtgtgtatg tttttttgct actgttatgt
     1861 tgacttattc tgttatttgg ttctgaatga tgtcaaacta atgggattat ttgtgggttt
     1921 gtacaggtgg ctctattcta tgatgatggt cgtcctgttg aacaaaaggg tgttggaaga
     1981 aaaatccttg acaaggtgca tcagacttac cattctgatc tggatggtaa agagtttgct
     2041 tatgacggtg agaagacgtt gtttacatat ggagctttgc ctagtaacaa gatggatttt
     2101 tctgtggtgc ttgaggaagt atctgctaca aggtaaaaca tcatctgact atagtcatta
     2161 cattggttgt gtgctttagt ttttgttctt agtaaggatt ttgtgagcag ggctaatgga
     2221 aacggaagcc ccaatgggaa tgaaagtcca agtgatggtg ataggaaaag actgcgtagg
     2281 cctaaccggt ccaaaaactt tagagtggag atcagctatg cggccaaaat tcctcttcaa
     2341 gctcttgcta atgcaatgcg gggacaagaa tcagagaatt cccaggaggc aatacgggtt
     2401 cttgatatca tattgaggca acatgctgct agacagtaag tgtcaagtgt atatatgtat
     2461 tttgttaatc tcatatattt gtctttctaa tacctatggt acaaattgca gaggttgctt
     2521 gcttgttcga cagtcttttt tccacaatga tccaaccaac tgtgaaccag ttggtggtaa
     2581 catcttagga tgtaggggat ttcactccag tttcagaaca acgcagggtg gcatgtcact
     2641 taatatgggt acataatttc acttgtactt gatgttggtg tttgattctg tctgttgtag
     2701 caataattta gtgtctaata gtttatgttt tgttcttatg ctagatgtta caaccaccat
     2761 gatcatcaag cctggtccag tggttgattt cctaattgct aaccaaaatg ctagggaccc
     2821 ttattcgatt gactggtcta aggttaacta cttattatga tatgtggctt tttcataatc
     2881 taaacatgtt ccttacctct tttgcttggc ttgacacatt ttaacatgtt gttttcaaca
     2941 ggctaaacga acccttaaga acctaagggt aaaggtcagc ccctcaggcc aagaattcaa
     3001 gataaccgga ttgagtgaca agccttgcag ggaacaaacg taagatattc actaatttga
     3061 ctttgttgtg agatgcattt gtttctgttt tagttttctg acgttgtgat ctctttcaaa
     3121 gataaatggt tctctgaagt ttgcttttta cactgtctag gtttgaattg aagaaaagga
     3181 acccaaatga aaatggagag ttcgaaacta ctgaagttac agttgctgac tacttccgcg
     3241 atacaaggca tattgatttg caatattctg cggatttgcc ttgcatcaat gttgggaagc
     3301 caaagcgacc cacttacatt cctctcgagg tttgcctctt atactcatca acgttctcat
     3361 agtcatggta ttaatttgtt ttcaaaaatc atgctaatgt atgtaatgtg gtggaatttt
     3421 cgcagctctg cgcgttggtt ccacttcaga ggtacacaaa agcacttacc acgttccaaa
     3481 gatctgccct tgttgagaaa tccagacaga aaccccaaga gaggatgact gttctgtcca
     3541 aagtaaagta tcctgaccgc ttcataactg ttctgttttg tcagtgaact ggaatttaca
     3601 tatctttggt ttcatgcttt caggctctga aagttagcaa ctatgatgcg gaaccactcc
     3661 tgcgatcctg tggcatttcg atcagctcca actttactca ggtggagggt cgtgttctac
     3721 cagctcccaa ggtatggtgc gatcgcttac tttttgacat gatgaattgt ttttagcttt
     3781 tgcatacatg taatattctc ttgttttctt tgactgacag ctgaaaatgg gatgtggatc
     3841 tgaaaccttt cccagaaatg gtcgctggaa cttcaacaac aaggtgctgt ttctctctgc
     3901 ctttctttgt attttacctg ttcagagagt ctgattccct ttgttgtaca ggaatttgtt
     3961 gagcccacca aaattcaacg atgggttgtt gtcaatttct ctgctcgctg taatgtacgt
     4021 caagttgttg atgatctgat aaaaattgga ggatcaaaag gaattgtaag caggattctc
     4081 ttcttttgtt tttagtattc ttgaaaactt gtttgtgatt atcaacaatt tagatactgc
     4141 ttctttctgt attctgtccc aggaaattgc ttctcccttt caagtgtttg aggagggtaa
     4201 tcaattccgc cgtgctcctc ctatgattcg tgttgagaac atgtttaagg acatccaatc
     4261 gaaactccct ggtgtcccac aattcatact atgtgtgctc cctgacaaaa agaacagtga
     4321 tctctatggt tagatatctc ttaaatccag tcttcaatca tctttttgtt ttatataata
     4381 gaagtttcct tggataaata ctttgaatcc ttcatcaggt ccatggaaga aaaaaaactt
     4441 aactgaattt ggcattgtta ctcaatgcat ggctccaacg cggcaaccta atgatcagta
     4501 tcttactaac ttacttctga agattaatgc aaaggtgatc tctctccttt tttctctttt
     4561 ggaaatggtt tttgcttctt gtgtgcaatc tctgataaca tataactaca gcttggaggc
     4621 ctgaactcaa tgttaagtgt agagcgtaca cctgcgttca ctgtgatttc taaggttcca
     4681 accattatcc ttgggatgga tgtttcacat ggatctcctg gacagtctga tgtcccgtcc
     4741 atcgctgctg taaaatgttt actcactgct gttgtttgct ttttagtttg gctagttctt
     4801 caagactgat atttagttgt ctcttttgtt atgattaact taggtggtga gttctaggga
     4861 gtggccactg atatccaaat atagagcatc tgttcggaca cagccttcta aggctgagat
     4921 gattgagtcc cttgtcaaga aaaatggaac tgaagacgat ggcattatca agtgtgcact
     4981 tacactaact cttgatgtgg cttttgttta atctttgcat gaagtctggt ttaagtatct
     5041 atgtaacttt atttgttgat tgcagggagt tgctggtaga tttctacacc agctcgaata
     5101 agagaaaacc agagcatatc ataattttca ggtatgttac atcagagctt ttgtctcttc
     5161 atttttccca tctttatttt ctcatattgg ttgtttcgat gtttaatgct tctagggatg
     5221 gtgtgagtga atctcaattc aatcaggttc tgaatattga acttgatcag atcatcgagg
     5281 tagttgaaac ttcaaaataa aagtttctca gtgtaatttt cttcttctgt tagtacttaa
     5341 ttgcatttct ctttcctcag gcttgcaagc tcttagacgc aaattggaac ccaaagttcc
     5401 ttttgttggt ggctcaaaag aatcatcata ccaagttctt ccagccaacg tctcctgaaa
     5461 atgttcctcc aggtgattga caaatctata ttataacctt ttttgacaca tagctgatct
     5521 ctatctcatg ggggttttgg ttattttgca gggacaatca ttgacaacaa aatatgtcac
     5581 ccaaagaaca atgatttcta cctctgtgct cacgctggaa tgattgtaag tttcatctat
     5641 caactcaaaa ctttatttgt taatgtttgc tcctctgaat cgttttaata ctctttatgt
     5701 tctgaattag ggaactaccc gcccaactca ctaccacgtc ctgtatgatg agattggttt
     5761 ttcagctgac gaacttcagg aacttgtcca ctcgctctcc tatgtgtatg tagtctctta
     5821 ttcaaccttc attacatttt ttgtgacaac ttcgattgtg atgataacag aaattatctg
     5881 atttctttct ttggcaaaat tccaggtacc aaagaagcac cagtgccatt tctgttggta
     5941 attaccataa ctgttttctc tactccatcg atttaaagtc gtcttcattt gttctcttaa
     6001 taactgacat tttaattttt tgtgcagttg cgccgatctg ctatgctcac ttggcagctg
     6061 ctcagcttgg gacgttcatg aagtttgaag atcagtctga gacatcatca agccatggtg
     6121 gtatcacagc tccaggacca atctctgttg cacagctccc aagactcaaa gacaacgtcg
     6181 ccaactccat gttcttctgt taactagata cccagctttc ttgtacaaag tggtgatatc
     6241 ccgcggccat ggcggccggg agcatgcgac gtcgatctaa ctgactagcc gcggccatgc
     6301 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     6361 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     6421 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     6481 aaaatttcta atccctaaaa ccaaaatcca gtgacctccc gggtaccgcg aattatcgat
     6541 catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg
     6601 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct
     6661 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
     6721 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
     6781 ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga ggggatctac
     6841 catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg acatgccggc
     6901 ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc gtaccgagcc
     6961 gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct atccctggct
     7021 cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggtccct ggaaggcacg
     7081 caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac
     7141 gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa
     7201 gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct
     7261 cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga
     7321 cgtgggtttc tggcagctgg acttcagcct gccggtgccg ccccgtccgg tcctgcccgt
     7381 caccgaaatc tgatgacccc tagagtcaag cagatcgttc aaacatttgg caataaagtt
     7441 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     7501 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     7561 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     7621 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaccg gcatgcaagc
     7681 tgataattca attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     7741 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     7801 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     7861 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     7921 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     7981 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     8041 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     8101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     8161 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     8221 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     8281 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     8341 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     8401 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     8461 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     8521 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     8581 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     8641 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     8701 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     8761 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     8821 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     8881 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     8941 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     9001 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     9061 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     9121 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     9181 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     9241 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     9301 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9361 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9421 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9481 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9541 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9601 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9661 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9721 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9781 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9841 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9901 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9961 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10021 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10081 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
    10141 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
    10201 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
    10261 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    10321 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    10381 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
    10441 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
    10501 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
    10561 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
    10621 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
    10681 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
    10741 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
    10801 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
    10861 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
    10921 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
    10981 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
    11041 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
    11101 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
    11161 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
    11221 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
    11281 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
    11341 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
    11401 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
    11461 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
    11521 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
    11581 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
    11641 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
    11701 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
    11761 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
    11821 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
    11881 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
    11941 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
    12001 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    12061 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    12121 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    12181 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    12241 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    12301 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    12361 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    12421 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    12481 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    12541 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    12601 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    12661 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    12721 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    12781 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    12841 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    12901 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    12961 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    13021 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    13081 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    13141 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    13201 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    13261 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    13321 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    13381 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    13441 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    13501 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    13561 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    13621 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    13681 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    13741 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    13801 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    13861 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    13921 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    13981 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    14041 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    14101 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    14161 ttgga
//
