LOCUS       sequence-435360-        2883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for high expression in 
            mammalian cells with an added 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.0
FEATURES             Location/Qualifiers
     source          1..2883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     630..646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        726..1105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1106..1309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1260..1280
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1354..1372
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1354..1371
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1605..1739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1642..1661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1696..1715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1809..1826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1809..1825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1825..1896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1897..1965,1966..2757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2115..2134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2758..2777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2758..2776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2865..2883)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaagggacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      781 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      841 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      901 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      961 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1021 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1081 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1141 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1201 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1261 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     1321 tagagaaccc actgcttact ggcttatcga aatatttagg tgacactata gagacccaag
     1381 cttggcattc cggtactgtt ggtaaaactc tcgaaacttg gctagctccc ctccgaagag
     1441 ccgagtgtct tcggttcgag accgaattcg cgagctctcg ctagccagtt taaacaattg
     1501 cggccgcgta ctagtctatc gatttggatc caccctgcag gaagtctaga ccgtcgacac
     1561 ggtctctgag agaagacacg tcgctcttcg acgtaagtaa ctgaaacttg tttattgcag
     1621 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1681 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     1741 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
     1801 taagcttaac tggccgtcgt tttacattca aatatgtatc cgctcatgag acaataaccc
     1861 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1921 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1981 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2041 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2101 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2161 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2221 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2281 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2341 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2401 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2461 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2521 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2581 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2641 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2701 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaataa
     2761 tacgactcac tatagggtaa ctaattgagg cgcgcctacc accgtcaaaa aaaacggcgc
     2821 tttttagcgc cgtttttatt tttcaacctt cctaggtaag tgacccttta gtgagggtta
     2881 att
//
