LOCUS       sequence-435420-        7908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac 
            integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Hanswillemenke A, Hofacker DT, Sorgenfrei M, Fruhner C, 
            Franz-Wachtel M, Schwarzer D, Macek B, Stafforst T
  TITLE     Profiling the interactome of oligonucleotide drugs by proximity 
            biotinylation.
  JOURNAL   Nat Chem Biol. 2024 Jan 17. doi: 10.1038/s41589-023-01530-z.
  PUBMED    38233583
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Jan 17. doi: 10.1038/s41589-023-01530-z."
COMMENT     Sequence Label: Xlone Puro SNAP-eGFP-BASU
FEATURES             Location/Qualifiers
     source          1..7908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             400..942
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             join(973..975,976..1686)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(973..975,976..1686)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1015..1036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1276..1295)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1623..1644
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2663..2797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3073..3107
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3118..3135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3118..3134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3127..3149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3343..3362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3462..3484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3522..3540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3608..3712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3713..3781,3782..4573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3931..3950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4744..5332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5233..5252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5486..5503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5620..5641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5656..5661,5662..5679,5680..5686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5694..5710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5699..5721
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5718..5734
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5718..5734
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5760..5822
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5998..6079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6202..6801)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6285..6305)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6782..6801
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6802..6858)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6868..7611)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7612..7635)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7635..7644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7664..7875)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgcggccgc atggccggcg acaaagactg cgaaatgaag
      421 cgcaccaccc tggatagccc tctgggcaag ctggaactgt ctgggtgcga acagggcctg
      481 caccgtatca tcttcctggg caaaggaaca tctgccgccg acgccgtgga agtgcctgcc
      541 ccagccgccg tgctgggcgg accagagcca ctgatgcagg ccaccgcctg gctcaacgcc
      601 tactttcacc agcctgaggc catcgaggag ttccctgtgc cagccctgca ccacccagtg
      661 ttccagcagg agagctttac ccgccaggtg ctgtggaaac tgctgaaagt ggtgaagttc
      721 ggagaggtca tcagctacag ccacctggcc gccctggccg gcaatcccgc cgccaccgcc
      781 gccgtgaaaa ccgccctgag cggaaatccc gtgcccattc tgatcccctg ccaccgggtg
      841 gtgcagggcg acctggacgt ggggggctac gagggcgggc tcgccgtgaa agagtggctg
      901 ctggcccacg agggccacag actgggcaag cctgggctgg gtcctgcagg cggaggcgcg
      961 ccagggtctg gcgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1021 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1081 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1141 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1201 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1261 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1321 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1381 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1441 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1501 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1561 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1621 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1681 tacaagccgg tcaccggcgg aaaacttagc gagagcgaga tccgcttcgg cctgaaaacc
     1741 gaagtgatgg gccagcacct gatctaccac gacgtgctga gcagcaccca gaaaacagcc
     1801 cacgagctgg ccaacaacaa tgcccctgag ggaacactgg tggtggccga taagcagaca
     1861 gctggccgcg gcggaatgag ccgcgtgtgg cacagccaag aaggcaacgg cgtctggatg
     1921 agcctgatcc tgcgccctga catccctctc cagaaaaccc ctcagctgac actgctggct
     1981 gctgtggctg tggtgcaggg aattgaagaa gccgccggaa tccagaccga catcaagtgg
     2041 cccaacgaca tcctgatcaa cggcaagaaa accgtgggca tcctgaccga gatgcaggcc
     2101 gaagaggaca gagtccgcag cgtgatcatc ggcatcggga tcaatgtgaa ccagcagcct
     2161 aacgacttcc ccgacgagct gaaggatatc gccacaagcc tgtctcaggc cgctggcgag
     2221 aagattgatc gcgctggcgt gatccagcac atcctgctgt gcttcgagaa gcggtaccgc
     2281 gactacatga cccacggctt cacccctatc aaactgctgt gggagagcta cgccctcgga
     2341 atcggcacca acatgcgcgc ccgcacactg aacggcacct tctacggcaa ggccctggga
     2401 attgatgacg agggcgttct gctgctggaa acaaacgagg gcatcaagaa aatctacagc
     2461 gccgacatca gcctgcgcta attctagtag accacctccc ctgcgagcta agctggacag
     2521 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
     2581 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
     2641 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
     2701 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
     2761 cttatttgta accattataa gctgcaataa acaagtttga tatctataac aagaaaatat
     2821 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     2881 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     2941 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     3001 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     3061 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta agaattcact
     3121 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3181 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3241 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     3301 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3361 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3421 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3481 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3541 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     5641 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5701 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagg tcgacttaac
     5761 cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa aattgacgca
     5821 tgtgttttat cggtctgtat atcgaggttt atttattaat ttgaatagat attaagtttt
     5881 attatattta cacttacata ctaataataa attcaacaaa caatttattt atgtttattt
     5941 atttattaaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttagca
     6001 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6061 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6121 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6181 tgatcctctg gagatcctag gctaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     6241 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     6301 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     6361 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     6421 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     6481 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     6541 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     6601 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     6661 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
     6721 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
     6781 cgtgggcttg tactcggtca tggggccggg gttctcctcc acgtcgccgg cctgcttcag
     6841 caggctgaag ttggtggcgc cgctgccccc ggggagcatg tcaaggtcaa aatcgtcaag
     6901 agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc
     6961 taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc
     7021 caggccacat atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc
     7081 gaacagctca attgcttgtt tcagaagtgg gggcatagaa tcggtggtag gtgtctctct
     7141 ttcctctttt gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc
     7201 cacggcggac agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc
     7261 gagctgattt tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt
     7321 agccccgtcg cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa
     7381 gtcttgccat gactcgcctt ccagggggca ggagtgggta tgatgcctgt ccagcatctc
     7441 gattggcagg gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg
     7501 ctcaactccc agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt
     7561 gagtaattcc agagcagagt ttatgacttt gctcttgtcc agtctagaca tcttatcgtc
     7621 atcgtctttg taatccatgg tggcggatcc cgcgtcacga cacctgtgtt ctggcggcaa
     7681 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     7741 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     7801 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
     7861 atgtgcgctc tgcccactga cgggcaccgg agccactcga gtggaatt
//
