LOCUS       sequence-43546-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA6c-RFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      147..218
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      234..261
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(289..306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
       61 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc
      121 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      181 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      241 cttcgggtgg gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga
      301 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
      361 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
      421 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
      541 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
      601 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
      661 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      721 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
      781 agttagtctt gaagtcatgc gccggttaaa gctaaactga aaggacaagt tttggtgact
      841 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
      901 aaccgccctg caaggcggtt ttttcgtttt
//
