LOCUS       sequence-435502-        8430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use 
            with MOBE systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC
  TITLE     Development of multiplexed orthogonal base editor (MOBE) systems.
  JOURNAL   Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0.
  PUBMED    38773305
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0."
COMMENT     Sequence Label: nCas9-SpRY_P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        270..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        650..853
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     804..824
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     895..914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        895..913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             963..983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             984..5084
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5127..5147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5157..5213
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5214..5921
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5359..5377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5627..5646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5934..5954
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5963..5980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6003..6020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6009..6233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6304..6320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6304..6320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6317..6339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6328..6344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6352..6358,6359..6376,6377..6382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6397..6418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6535..6552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6706..7294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6786..6805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7465..8256,8257..8325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8088..8107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8326..8430)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac
      901 gactcactat agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt
      961 caccaaagaa gaagcggaaa gtcgacaaga agtacagcat cggcctggcc atcggcacca
     1021 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     1081 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
     1141 acagcggcga aacagccgag agaacccggc tgaagagaac cgccagaaga agatacacca
     1201 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     1261 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     1321 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     1381 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     1441 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     1501 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     1561 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     1621 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     1681 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     1741 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     1801 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1861 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1921 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1981 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     2041 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     2101 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     2161 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     2221 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     2281 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     2341 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     2401 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     2461 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     2521 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     2581 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     2641 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     2701 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     2761 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     2821 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2881 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2941 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     3001 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     3061 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     3121 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     3181 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     3241 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     3301 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     3361 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     3421 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     3481 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca
     3541 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     3601 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     3661 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     3721 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     3781 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     3841 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3901 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3961 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     4021 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     4081 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     4141 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     4201 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     4261 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc agacccaaga
     4321 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     4381 tcctgtggcc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     4441 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     4501 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     4561 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     4621 tggcctctgc caagcagctg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     4681 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     4741 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     4801 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     4861 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     4921 ccctgaccag actgggagcc cctagagcct tcaagtactt tgacaccacc atcgacccca
     4981 agcagtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     5041 gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctggc ggctcaaaaa
     5101 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga agcggagcta
     5161 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga
     5221 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     5281 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5341 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     5401 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     5461 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5521 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     5581 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     5641 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     5701 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     5761 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     5821 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     5881 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtctggtggt tctcccaaga
     5941 agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     6001 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6061 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6121 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6181 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6241 ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc
     6301 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6361 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7141 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7321 gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8401 ttagaaaaat aaacaaatag gggttccgcg
//
