LOCUS       sequence-43551-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS6a-RFP
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    208..224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    231..247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(315..334)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        540..617
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(637..656)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg
       61 aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata
      121 acgtcttcgc tactcgccat atgtatatct ccttcttaaa agatcttttg aattcggtca
      181 gtgcgtcctg ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg
      241 ctcacaattc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      301 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      361 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      421 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      481 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
      541 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
      601 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
      661 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
      721 gggaaaaagt gggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      781 caac
//
